Comparative evaluation of the QUANTIPLEX HIV-1 RNA 2.0 and 3.0 (bDNA) assays and the AMPLICOR HIV-1 MONITOR v1.5 test for the quantitation of human immunodeficiency virus type 1 RNA in plasma

HIV-1 RNA measurements from 84 plasma specimens obtained with the QUANTIPLEX HIV-1 RNA 2.0 and 3.0 (bDNA) assays (Chiron Diagnostics, Emeryville, CA) and with the AMPLICOR HIV-1 MONITOR™ Test, version 1.5 with ultra-sensitive specimen preparation (Roche Diagnostic Systems, Inc., Branchburg, NJ) were...

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Veröffentlicht in:	Journal of virological methods 2001, Vol.91 (1), p.67-74
Hauptverfasser:	Anastassopoulou, Cleo G, Touloumi, Giota, Katsoulidou, Antigoni, Hatzitheodorou, Helen, Pappa, Maroudia, Paraskevis, Dimitrios, Lazanas, Marios, Gargalianos, Panagiotis, Hatzakis, Angelos
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult Amplicor Biological and medical sciences Branched DNA Branched DNA Signal Amplification Assay DNA Probes Female Fundamental and applied biological sciences. Psychology HIV Infections - virology HIV-1 - genetics HIV-1 - isolation & purification HIV-1 RNA Human immunodeficiency virus 1 Humans Male Microbiology Quantiplex assay Reagent Kits, Diagnostic Reverse Transcriptase Polymerase Chain Reaction RNA levels RNA, Viral - blood Viral Load
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Zusammenfassung:	HIV-1 RNA measurements from 84 plasma specimens obtained with the QUANTIPLEX HIV-1 RNA 2.0 and 3.0 (bDNA) assays (Chiron Diagnostics, Emeryville, CA) and with the AMPLICOR HIV-1 MONITOR™ Test, version 1.5 with ultra-sensitive specimen preparation (Roche Diagnostic Systems, Inc., Branchburg, NJ) were compared. The absolute RNA values of tested specimens differed significantly between bDNA 2.0 and bDNA 3.0 or Monitor v1.5 measurements (Wilcoxon signed-rank test P
ISSN:	0166-0934 1879-0984
DOI:	10.1016/S0166-0934(00)00245-7