The activation of platinum(II) antiproliferative drugs in carbonate medium evaluated by means of a DNA-biosensor

We report on the binding of cisplatin, carboplatin and oxaliplatin to double-stranded DNA in two different (phosphate and carbonate) buffers, using an electrochemical DNA-biosensor. The propensity of the electrophilic agent produced by hydrolysis to interact with DNA was measured as a function of th...

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Veröffentlicht in:	Journal of inorganic biochemistry 2007-07, Vol.101 (7), p.1023-1027
Hauptverfasser:	Ravera, Mauro, Bagni, Graziana, Mascini, Marco, Dabrowiak, James C., Osella, Domenico
Format:	Artikel
Sprache:	eng
Schlagworte:	Antineoplastic Agents - chemistry Biosensing Techniques - methods Carbonate buffer Carbonates - chemistry Carboplatin - chemistry Cisplatin - chemistry DNA - chemistry DNA-biosensor Hydrogen-Ion Concentration Molecular Structure Organoplatinum Compounds - chemistry Platinum drugs Time Factors
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Beschreibung
Zusammenfassung:	We report on the binding of cisplatin, carboplatin and oxaliplatin to double-stranded DNA in two different (phosphate and carbonate) buffers, using an electrochemical DNA-biosensor. The propensity of the electrophilic agent produced by hydrolysis to interact with DNA was measured as a function of the decrease of guanine oxidation signal of the metal–DNA adduct immobilized on a screen-printed electrode, by using square wave voltammetry. The results obtained confirm that carbonate reacts with platinum drugs to form activated carbonato complexes, which are able to react readily with DNA.
ISSN:	0162-0134 1873-3344
DOI:	10.1016/j.jinorgbio.2007.03.017