High-throughput mapping of cell-wall polymers within and between plants using novel microarrays

We describe here a methodology that enables the occurrence of cell-wall glycans to be systematically mapped throughout plants in a semi-quantitative high-throughput fashion. The technique (comprehensive microarray polymer profiling, or CoMPP) integrates the sequential extraction of glycans from mult...

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Veröffentlicht in:The Plant journal : for cell and molecular biology 2007-06, Vol.50 (6), p.1118-1128
Hauptverfasser: Moller, Isabel, Sørensen, Iben, Bernal, Adriana J, Blaukopf, Claudia, Lee, Kieran, Øbro, Jens, Pettolino, Filomena, Roberts, Alison, Mikkelsen, Jørn Dalgaard, Knox, J. Paul, Bacic, Antony, Willats, William G.T
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container_end_page 1128
container_issue 6
container_start_page 1118
container_title The Plant journal : for cell and molecular biology
container_volume 50
creator Moller, Isabel
Sørensen, Iben
Bernal, Adriana J
Blaukopf, Claudia
Lee, Kieran
Øbro, Jens
Pettolino, Filomena
Roberts, Alison
Mikkelsen, Jørn Dalgaard
Knox, J. Paul
Bacic, Antony
Willats, William G.T
description We describe here a methodology that enables the occurrence of cell-wall glycans to be systematically mapped throughout plants in a semi-quantitative high-throughput fashion. The technique (comprehensive microarray polymer profiling, or CoMPP) integrates the sequential extraction of glycans from multiple organs or tissues with the generation of microarrays, which are probed with monoclonal antibodies (mAbs) or carbohydrate-binding modules (CBMs) with specificities for cell-wall components. The profiles generated provide a global snapshot of cell-wall composition, and also allow comparative analysis of mutant and wild-type plants, as demonstrated here for the Arabidopsis thaliana mutants fra8, mur1 and mur3. CoMPP was also applied to Physcomitrella patens cell walls and was validated by carbohydrate linkage analysis. These data provide new insights into the structure and functions of plant cell walls, and demonstrate the potential of CoMPP as a component of systems-based approaches to cell-wall biology.
doi_str_mv 10.1111/j.1365-313x.2007.03114.x
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subjects Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis thaliana
Biological and medical sciences
Bryopsida - metabolism
carbohydrate binding modules
Carbohydrate Conformation
Cell physiology
Cell Wall - metabolism
Cells, cell elements: structure and function
Fundamental and applied biological sciences. Psychology
glycan arrays
monoclonal antibodies
Mutation
Physcomitrella patens
plant cell walls
Plant physiology and development
Polysaccharides - metabolism
Tissue Array Analysis - methods
title High-throughput mapping of cell-wall polymers within and between plants using novel microarrays
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