High-throughput mapping of cell-wall polymers within and between plants using novel microarrays
We describe here a methodology that enables the occurrence of cell-wall glycans to be systematically mapped throughout plants in a semi-quantitative high-throughput fashion. The technique (comprehensive microarray polymer profiling, or CoMPP) integrates the sequential extraction of glycans from mult...
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Veröffentlicht in: | The Plant journal : for cell and molecular biology 2007-06, Vol.50 (6), p.1118-1128 |
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creator | Moller, Isabel Sørensen, Iben Bernal, Adriana J Blaukopf, Claudia Lee, Kieran Øbro, Jens Pettolino, Filomena Roberts, Alison Mikkelsen, Jørn Dalgaard Knox, J. Paul Bacic, Antony Willats, William G.T |
description | We describe here a methodology that enables the occurrence of cell-wall glycans to be systematically mapped throughout plants in a semi-quantitative high-throughput fashion. The technique (comprehensive microarray polymer profiling, or CoMPP) integrates the sequential extraction of glycans from multiple organs or tissues with the generation of microarrays, which are probed with monoclonal antibodies (mAbs) or carbohydrate-binding modules (CBMs) with specificities for cell-wall components. The profiles generated provide a global snapshot of cell-wall composition, and also allow comparative analysis of mutant and wild-type plants, as demonstrated here for the Arabidopsis thaliana mutants fra8, mur1 and mur3. CoMPP was also applied to Physcomitrella patens cell walls and was validated by carbohydrate linkage analysis. These data provide new insights into the structure and functions of plant cell walls, and demonstrate the potential of CoMPP as a component of systems-based approaches to cell-wall biology. |
doi_str_mv | 10.1111/j.1365-313x.2007.03114.x |
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Paul ; Bacic, Antony ; Willats, William G.T</creator><creatorcontrib>Moller, Isabel ; Sørensen, Iben ; Bernal, Adriana J ; Blaukopf, Claudia ; Lee, Kieran ; Øbro, Jens ; Pettolino, Filomena ; Roberts, Alison ; Mikkelsen, Jørn Dalgaard ; Knox, J. Paul ; Bacic, Antony ; Willats, William G.T</creatorcontrib><description>We describe here a methodology that enables the occurrence of cell-wall glycans to be systematically mapped throughout plants in a semi-quantitative high-throughput fashion. The technique (comprehensive microarray polymer profiling, or CoMPP) integrates the sequential extraction of glycans from multiple organs or tissues with the generation of microarrays, which are probed with monoclonal antibodies (mAbs) or carbohydrate-binding modules (CBMs) with specificities for cell-wall components. The profiles generated provide a global snapshot of cell-wall composition, and also allow comparative analysis of mutant and wild-type plants, as demonstrated here for the Arabidopsis thaliana mutants fra8, mur1 and mur3. CoMPP was also applied to Physcomitrella patens cell walls and was validated by carbohydrate linkage analysis. These data provide new insights into the structure and functions of plant cell walls, and demonstrate the potential of CoMPP as a component of systems-based approaches to cell-wall biology.</description><identifier>ISSN: 0960-7412</identifier><identifier>EISSN: 1365-313X</identifier><identifier>DOI: 10.1111/j.1365-313x.2007.03114.x</identifier><identifier>PMID: 17565618</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>Arabidopsis - genetics ; Arabidopsis - metabolism ; Arabidopsis thaliana ; Biological and medical sciences ; Bryopsida - metabolism ; carbohydrate binding modules ; Carbohydrate Conformation ; Cell physiology ; Cell Wall - metabolism ; Cells, cell elements: structure and function ; Fundamental and applied biological sciences. Psychology ; glycan arrays ; monoclonal antibodies ; Mutation ; Physcomitrella patens ; plant cell walls ; Plant physiology and development ; Polysaccharides - metabolism ; Tissue Array Analysis - methods</subject><ispartof>The Plant journal : for cell and molecular biology, 2007-06, Vol.50 (6), p.1118-1128</ispartof><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5684-5e5dfbf555744ad502e1640c6b93b55c579b1046284553a093afab81c8b9ab893</citedby><cites>FETCH-LOGICAL-c5684-5e5dfbf555744ad502e1640c6b93b55c579b1046284553a093afab81c8b9ab893</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-313X.2007.03114.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-313X.2007.03114.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18807881$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17565618$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Moller, Isabel</creatorcontrib><creatorcontrib>Sørensen, Iben</creatorcontrib><creatorcontrib>Bernal, Adriana J</creatorcontrib><creatorcontrib>Blaukopf, Claudia</creatorcontrib><creatorcontrib>Lee, Kieran</creatorcontrib><creatorcontrib>Øbro, Jens</creatorcontrib><creatorcontrib>Pettolino, Filomena</creatorcontrib><creatorcontrib>Roberts, Alison</creatorcontrib><creatorcontrib>Mikkelsen, Jørn Dalgaard</creatorcontrib><creatorcontrib>Knox, J. Paul</creatorcontrib><creatorcontrib>Bacic, Antony</creatorcontrib><creatorcontrib>Willats, William G.T</creatorcontrib><title>High-throughput mapping of cell-wall polymers within and between plants using novel microarrays</title><title>The Plant journal : for cell and molecular biology</title><addtitle>Plant J</addtitle><description>We describe here a methodology that enables the occurrence of cell-wall glycans to be systematically mapped throughout plants in a semi-quantitative high-throughput fashion. The technique (comprehensive microarray polymer profiling, or CoMPP) integrates the sequential extraction of glycans from multiple organs or tissues with the generation of microarrays, which are probed with monoclonal antibodies (mAbs) or carbohydrate-binding modules (CBMs) with specificities for cell-wall components. The profiles generated provide a global snapshot of cell-wall composition, and also allow comparative analysis of mutant and wild-type plants, as demonstrated here for the Arabidopsis thaliana mutants fra8, mur1 and mur3. CoMPP was also applied to Physcomitrella patens cell walls and was validated by carbohydrate linkage analysis. These data provide new insights into the structure and functions of plant cell walls, and demonstrate the potential of CoMPP as a component of systems-based approaches to cell-wall biology.</description><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis thaliana</subject><subject>Biological and medical sciences</subject><subject>Bryopsida - metabolism</subject><subject>carbohydrate binding modules</subject><subject>Carbohydrate Conformation</subject><subject>Cell physiology</subject><subject>Cell Wall - metabolism</subject><subject>Cells, cell elements: structure and function</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>glycan arrays</subject><subject>monoclonal antibodies</subject><subject>Mutation</subject><subject>Physcomitrella patens</subject><subject>plant cell walls</subject><subject>Plant physiology and development</subject><subject>Polysaccharides - metabolism</subject><subject>Tissue Array Analysis - methods</subject><issn>0960-7412</issn><issn>1365-313X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAQhi0EokvhL4AvcEuYiT_iHDigClpQJZBoJW6Wk3V2vXI-sBN299_XYRf1CL6MJT-vZ953CKEIOabzfpcjkyJjyA55AVDmwBB5fnhCVn8ffj4lK6gkZCXH4oK8iHEHgCWT_Dm5wFJIIVGtiL5xm202bcMwb7bjPNHOjKPrN3RoaWO9z_bGezoO_tjZEOneTVvXU9OvaW2nvbU9Hb3pp0jnuKj64bf1tHNNGEwI5hhfkmet8dG-OtdLcv_5093VTXb77frL1cfbrBFS8UxYsW7rVghRcm7WAgqLkkMj64rVQjSirGoELgvFhWAGKmZaUytsVF2lWrFL8u707xiGX7ONk-5cXAyY3g5z1CVIUGXy_y-wAIGY5kigOoHJS4zBtnoMrjPhqBH0sgW900vYeglbL1vQf7agD0n6-txjrju7fhSeY0_A2zNgYmN8G0zfuPjIKQWlUpi4Dydu77w9_vcA-u771-WW9G9O-tYM2mxC6nH_owBkCVYomGIPqMWs-A</recordid><startdate>200706</startdate><enddate>200706</enddate><creator>Moller, Isabel</creator><creator>Sørensen, Iben</creator><creator>Bernal, Adriana J</creator><creator>Blaukopf, Claudia</creator><creator>Lee, Kieran</creator><creator>Øbro, Jens</creator><creator>Pettolino, Filomena</creator><creator>Roberts, Alison</creator><creator>Mikkelsen, Jørn Dalgaard</creator><creator>Knox, J. Paul</creator><creator>Bacic, Antony</creator><creator>Willats, William G.T</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><general>Blackwell Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>200706</creationdate><title>High-throughput mapping of cell-wall polymers within and between plants using novel microarrays</title><author>Moller, Isabel ; Sørensen, Iben ; Bernal, Adriana J ; Blaukopf, Claudia ; Lee, Kieran ; Øbro, Jens ; Pettolino, Filomena ; Roberts, Alison ; Mikkelsen, Jørn Dalgaard ; Knox, J. 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Psychology</topic><topic>glycan arrays</topic><topic>monoclonal antibodies</topic><topic>Mutation</topic><topic>Physcomitrella patens</topic><topic>plant cell walls</topic><topic>Plant physiology and development</topic><topic>Polysaccharides - metabolism</topic><topic>Tissue Array Analysis - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Moller, Isabel</creatorcontrib><creatorcontrib>Sørensen, Iben</creatorcontrib><creatorcontrib>Bernal, Adriana J</creatorcontrib><creatorcontrib>Blaukopf, Claudia</creatorcontrib><creatorcontrib>Lee, Kieran</creatorcontrib><creatorcontrib>Øbro, Jens</creatorcontrib><creatorcontrib>Pettolino, Filomena</creatorcontrib><creatorcontrib>Roberts, Alison</creatorcontrib><creatorcontrib>Mikkelsen, Jørn Dalgaard</creatorcontrib><creatorcontrib>Knox, J. 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subjects | Arabidopsis - genetics Arabidopsis - metabolism Arabidopsis thaliana Biological and medical sciences Bryopsida - metabolism carbohydrate binding modules Carbohydrate Conformation Cell physiology Cell Wall - metabolism Cells, cell elements: structure and function Fundamental and applied biological sciences. Psychology glycan arrays monoclonal antibodies Mutation Physcomitrella patens plant cell walls Plant physiology and development Polysaccharides - metabolism Tissue Array Analysis - methods |
title | High-throughput mapping of cell-wall polymers within and between plants using novel microarrays |
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