Dynamic speckle illumination microscopy with wavelet prefiltering

Dynamic speckle illumination (DSI) provides a simple and robust technique to obtain fluorescence depth sectioning with a widefield microscope. We report a significant improvement to DSI microscopy based on a statistical image-processing algorithm that incorporates spatial wavelet prefiltering. The r...

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Veröffentlicht in:	Optics letters 2007-06, Vol.32 (11), p.1417-1419
Hauptverfasser:	VENTALON, Cathie, HEINTZMANN, Rainer, MERTZ, Jerome
Format:	Artikel
Sprache:	eng
Schlagworte:	Algorithms Animals Axons - metabolism Biological and medical sciences Conventional optical microscopes Exact sciences and technology Fundamental and applied biological sciences. Psychology General aspects, investigation technics, apparatus Green Fluorescent Proteins - chemistry Green Fluorescent Proteins - metabolism Hydrogen-Ion Concentration Image Processing, Computer-Assisted - methods Instruments, apparatus, components and techniques common to several branches of physics and astronomy Light Mice Microscopy Microscopy, Confocal Microscopy, Fluorescence - methods Models, Statistical Olfactory Bulb - metabolism Optical instruments, equipment and techniques Optics and Photonics Pattern Recognition, Automated Physics Tissues, organs and organisms biophysics
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Zusammenfassung:	Dynamic speckle illumination (DSI) provides a simple and robust technique to obtain fluorescence depth sectioning with a widefield microscope. We report a significant improvement to DSI microscopy based on a statistical image-processing algorithm that incorporates spatial wavelet prefiltering. The resultant gain in sectioning strength leads to a fundamentally improved scaling law for the out-of-focus background rejection.
ISSN:	0146-9592 1539-4794
DOI:	10.1364/OL.32.001417