Protocols for growth and development of Dictyostelium discoideum

Dictyostelium discoideum , a unicellular organism capable of developing into a multicellular structure, is a powerful model system to study a variety of biological processes. Because it is inexpensive and relatively easy to grow, Dictyostelium is also frequently used in teaching laboratories. Here w...

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Veröffentlicht in:	Nature protocols 2007-06, Vol.2 (6), p.1307-1316
Hauptverfasser:	Chisholm, Rex L, Fey, Petra, Kowal, Anthony S, Gaudet, Pascale, Pilcher, Karen E
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical Chemistry Animals Bacteria Biological Techniques Biomedical and Life Sciences Cell Movement Computational Biology/Bioinformatics Culture Techniques - methods Dictyostelium Dictyostelium - cytology Dictyostelium - growth & development Dictyostelium discoideum Laboratories Life Sciences Medical protocols Microarrays Morphogenesis Organic Chemistry Phototropism Physiological aspects protocol
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Beschreibung
Zusammenfassung:	Dictyostelium discoideum , a unicellular organism capable of developing into a multicellular structure, is a powerful model system to study a variety of biological processes. Because it is inexpensive and relatively easy to grow, Dictyostelium is also frequently used in teaching laboratories. Here we describe conditions for successfully growing and developing Dictyostelium cells and methods for long-term storage of Dictyostelium amoebae and spores.
ISSN:	1754-2189 1750-2799
DOI:	10.1038/nprot.2007.178