Leeia oryzae gen. nov., sp. nov., isolated from a rice field in Korea

1 Korea Research Institute of Bioscience and Biotechnology, 52 Oeundong, Yusong, Daejeon 305-333, Republic of Korea 2 Division of Applied Life Science, EB-NCRC, PMBBRC, Gyeongsang National University, Jinju 660-701, Republic of Korea 3 Yeongnam Agricultural Research Institute, 1085 Neidong, Milyang 627-803, Republic of Korea Correspondence Chang-Jin Kim changjin{at}kribb.re.kr A strictly aerobic, non-spore-forming, Gram-negative bacterium, designated strain HW7 T , was isolated from a rice field in Korea. Cells of strain HW7 T were short rod-shaped and motile with single polar flagella. The major cellular fatty acids of strain HW7 T were C 16 : 0 and summed feature 3 (C 16 : 1 7 c and/or iso-C 15 : 0 2-OH). The genomic DNA G+C content was 56 mol% and the major isoprenoid quinone was Q-8. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain HW7 T forms a distinct lineage with respect to closely related genera within the class Betaproteobacteria and that the levels of 16S rRNA gene sequence similarity with respect to the type species of related genera are less than 93 %. On the basis of the physiological and phylogenetic data, strain HW7 T represents a novel genus and species of the Betaproteobacteria , for which the name Leeia oryzae gen. nov., sp. nov. is proposed. The type strain is HW7 T (=KCTC 12585 T =DSM 17879 T ). The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain HW7 T is DQ280369 . Figures showing the general morphology and the polar lipids of cells of strain HW7 T are available as supplementary material with the online version of this paper. These authors contributed equally to this work.