Characterization of a Novel Triphosphonooctaosylceramide from the Eggs of the Sea Hare, Aplysia kurodai

We have reported the existence of a triphosphonoglycosphingolipid, EGL-I, in the eggs of a sea gastropod, Aplysia kurodai [Yamada, S., Araki, S., Abe, S., Kon, K., Ando, S., and Satake, M. (1995) J. Biochem. 117, 794–799]. We have now isolated a novel glycosphingolipid, named EGL-II, from the eggs o...

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Veröffentlicht in:Journal of biochemistry (Tokyo) 2001-01, Vol.129 (1), p.93-100
Hauptverfasser: Araki, Shigeko, Yamada, Shoji, Abe, Sachiko, Waki, Hatsue, Kon, Kazuo, Itonori, Saki, Sugita, Mutsumi, Ando, Susumu
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Sprache:eng
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Zusammenfassung:We have reported the existence of a triphosphonoglycosphingolipid, EGL-I, in the eggs of a sea gastropod, Aplysia kurodai [Yamada, S., Araki, S., Abe, S., Kon, K., Ando, S., and Satake, M. (1995) J. Biochem. 117, 794–799]. We have now isolated a novel glycosphingolipid, named EGL-II, from the eggs of Aplysia. By component analysis, sugar analysis, permethylation studies, fast atom bombardment—mass spectrometry, secondary ion mass spectrometry, and proton magnetic resonance spectrometry, its structure was revealed to be as follows: Galα1→3(GlcNAcα1→2)Galα1→3(3-O-MeGalα1→2)Galα1→3[6′-O-(2-aminoethylphosphonyl) Galα1→2](2-aminoethylphosphonyl→6)Galβ1→4(2-amino-ethylphosphonyl→6)Glcβ1→1ceramide. The major aliphatic components of the ceramide are palmitic acid, stearic acid, and anteisononadeca-4-sphingenine.
ISSN:0021-924X
DOI:10.1093/oxfordjournals.jbchem.a002841