Cellulomonas composti sp. nov., a cellulolytic bacterium isolated from cattle farm compost

1 Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Guseong-dong 373-1, Yuseong-gu, Daejeon 305-701, Republic of Korea 2 Department of Oriental Medicinal Materials and Processing, College of Life Science, Kyung Hee University, 1 Seocheon, Kihung Yongin, Kyunggi 449-701, Republic of Korea 3 School of Biology, King George VI Building, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK 4 Mount On Science Co. Ltd, Guseong-dong 373-1, Yuseong-gu, Daejeon 305-701, Republic of Korea Correspondence Sung-Taik Lee e_stlee{at}kaist.ac.kr A bacterial strain, TR7-06 T , which has cellulase and -glucosidase activities, was isolated from compost at a cattle farm near Daejeon, Republic of Korea. It was a Gram-positive, aerobic or facultatively anaerobic, non-motile, rod-shaped bacterium. Phylogenetic analysis based on 16S rRNA gene sequences showed that this strain belongs to the genus Cellulomonas , with highest sequence similarity to Cellulomonas uda DSM 20107 T (98.5 %). Cell wall analysis revealed the presence of type A4 , L -orn– D -Glu peptidoglycan. The cell-wall sugars detected were mannose and glucose. The predominant menaquinone was MK-9(H 4 ); MK-8(H 4 ) was detected in smaller quantities. The major fatty acids were anteiso-C 15 : 0 , C 16 : 0 , C 14 : 0 and C 18 : 0 . The polar lipids detected were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The results of DNA–DNA hybridization and physiological and biochemical tests clearly demonstrated that TR7-06 T represents a novel species. The combined genotypic and phenotypic data show that strain TR7-06 T (=KCTC 19030 T =NBRC 100758 T ) merits description as the type strain of a novel Cellulomonas species, Cellulomonas composti sp. nov. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain TR7-06 T is AB166887 . API ID32 test results for strain TR7-06 T and related Cellulomonas strains are available with the online version of this paper.