Aquaporin subtypes in rat cerebral microvessels

We investigated the expression of aquaporin (AQP) subtypes in the rat cerebral microvessels by reverse transcription-polymerase chain reaction, immunoblotting and immunohistochemistry. mRNA for AQP4, but not for AQP1, 2, 3 or 5, was detected in the microvessels. Immunoblot analysis showed that AQP4...

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Veröffentlicht in:	Neuroscience letters 2001-01, Vol.297 (3), p.163-166
Hauptverfasser:	Kobayashi, Hideyuki, Minami, Shin-ichi, Itoh, Satoru, Shiraishi, Seiji, Yokoo, Hiroki, Yanagita, Toshihiko, Uezono, Yasuhito, Mohri, Motohiko, Wada, Akihiko
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Aquaporin Aquaporin 1 Aquaporin 2 Aquaporin 3 Aquaporin 4 Aquaporin 5 Aquaporin 6 Aquaporins - biosynthesis Aquaporins - classification Aquaporins - genetics Biological and medical sciences Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges Cerebral Cortex - blood supply Cerebral Cortex - cytology Cerebral Cortex - metabolism Cerebral microvessels Fundamental and applied biological sciences. Psychology Immunohistochemistry Male Membrane Proteins Microcirculation - cytology Microcirculation - metabolism Organ Specificity Polymerase Chain Reaction Rats Rats, Sprague-Dawley Reverse transcription-polymerase chain reaction RNA, Messenger - biosynthesis Vertebrates: nervous system and sense organs Water permeability
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Zusammenfassung:	We investigated the expression of aquaporin (AQP) subtypes in the rat cerebral microvessels by reverse transcription-polymerase chain reaction, immunoblotting and immunohistochemistry. mRNA for AQP4, but not for AQP1, 2, 3 or 5, was detected in the microvessels. Immunoblot analysis showed that AQP4 protein was detected as a 30 kDa band with higher molecular weight bands. Immunohistochemical staining showed that AQP4 was located on cell surface of the cerebral microvessels. These results suggest that AQP4 in the cerebral microvessels is involved in the regulation of water transport between blood and brain.
ISSN:	0304-3940 1872-7972
DOI:	10.1016/S0304-3940(00)01705-5