Quantum Dot/Bioluminescence Resonance Energy Transfer Based Highly Sensitive Detection of Proteases
Sensing by BRET: Quantum dot (QD) nanosensors can detect the activity of matrix metalloproteinases by measuring the bioluminescence resonance energy transfer (BRET) efficiency between the QDs and a bioluminescent fusion protein (see scheme; Luc8=Renilla luciferase; His×6=six‐histidine tag).
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| Veröffentlicht in: | Angewandte Chemie International Edition 2007-01, Vol.46 (23), p.4346-4349 |
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| container_issue | 23 |
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| container_title | Angewandte Chemie International Edition |
| container_volume | 46 |
| creator | Yao, Hequan Zhang, Yan Xiao, Fei Xia, Zuyong Rao, Jianghong |
| description | Sensing by BRET: Quantum dot (QD) nanosensors can detect the activity of matrix metalloproteinases by measuring the bioluminescence resonance energy transfer (BRET) efficiency between the QDs and a bioluminescent fusion protein (see scheme; Luc8=Renilla luciferase; His×6=six‐histidine tag). |
| doi_str_mv | 10.1002/anie.200700280 |
| format | Article |
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| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | biosensors BRET (bioluminescence resonance energy transfer) Enzyme Activation Fluorescence Resonance Energy Transfer - methods luciferase Luminescent Measurements - methods Matrix Metalloproteinases - analysis Matrix Metalloproteinases - chemistry proteases Quantum Dots Sensitivity and Specificity |
| title | Quantum Dot/Bioluminescence Resonance Energy Transfer Based Highly Sensitive Detection of Proteases |
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