Quantum Dot/Bioluminescence Resonance Energy Transfer Based Highly Sensitive Detection of Proteases

Sensing by BRET: Quantum dot (QD) nanosensors can detect the activity of matrix metalloproteinases by measuring the bioluminescence resonance energy transfer (BRET) efficiency between the QDs and a bioluminescent fusion protein (see scheme; Luc8=Renilla luciferase; His×6=six‐histidine tag).

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Bibliographische Detailangaben
Veröffentlicht in:	Angewandte Chemie International Edition 2007-01, Vol.46 (23), p.4346-4349
Hauptverfasser:	Yao, Hequan, Zhang, Yan, Xiao, Fei, Xia, Zuyong, Rao, Jianghong
Format:	Artikel
Sprache:	eng
Schlagworte:	biosensors BRET (bioluminescence resonance energy transfer) Enzyme Activation Fluorescence Resonance Energy Transfer - methods luciferase Luminescent Measurements - methods Matrix Metalloproteinases - analysis Matrix Metalloproteinases - chemistry proteases Quantum Dots Sensitivity and Specificity
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Beschreibung
Zusammenfassung:	Sensing by BRET: Quantum dot (QD) nanosensors can detect the activity of matrix metalloproteinases by measuring the bioluminescence resonance energy transfer (BRET) efficiency between the QDs and a bioluminescent fusion protein (see scheme; Luc8=Renilla luciferase; His×6=six‐histidine tag).
ISSN:	1433-7851 1521-3773
DOI:	10.1002/anie.200700280