Probing a Fibrillation Nucleus Directly by Deep Ultraviolet Raman Spectroscopy

Amyloid fibril depositions are associated with many neurodegenerative diseases. The nucleation step of fibrillation remains poorly characterized as no experimental technique allows for direct monitoring of nucleus formation. A new method based on 2D-correlation deep UV resonance Raman spectroscopy w...

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Veröffentlicht in:	Journal of the American Chemical Society 2007-06, Vol.129 (22), p.6972-6973
Hauptverfasser:	Shashilov, Victor, Xu, Ming, Ermolenkov, Vladimir V, Fredriksen, Laura, Lednev, Igor K
Format:	Artikel
Sprache:	eng
Schlagworte:	Amyloid - chemistry Amyloid - metabolism Egg Proteins - chemistry Egg Proteins - metabolism Least-Squares Analysis Muramidase - chemistry Muramidase - metabolism Spectrophotometry, Ultraviolet - methods Spectrum Analysis, Raman - methods
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Zusammenfassung:	Amyloid fibril depositions are associated with many neurodegenerative diseases. The nucleation step of fibrillation remains poorly characterized as no experimental technique allows for direct monitoring of nucleus formation. A new method based on 2D-correlation deep UV resonance Raman spectroscopy was applied to probe directly all species at early stages of lysozyme fibrillation, establish the sequential order of highly correlated events, and characterize quantitatively the kinetics of nucleus formation.
ISSN:	0002-7863 1520-5126
DOI:	10.1021/ja070038c