Development of a novel functional biosensor with a short Ca(2+)-dependent deoxyribozyme

Development of a novel functional biosensor with a short Ca(2+)-dependent deoxyribozyme

We develop a novel functional biosensor on a deoxyribozyme. A 5'-end-immobilized short Ca(2+)-dependent deoxyribozyme (dCGCTGGCAGGCTACAACGAGTCTTC) binds to a target RNA substrate (rGAAGACA decrease UGCCAGCG; decrease denotes an RNA cleavage site), and acts as an enzyme in the presence of Ca2+....

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Nucleic acids symposium series (1979) 2000 (44), p.79-80
Hauptverfasser: Okumoto, Y, Sugimoto, N
Format: Artikel
Sprache:eng
Schlagworte:
Base Sequence
Binding Sites
Biosensing Techniques - methods
Calcium - metabolism
DNA, Catalytic - chemistry
DNA, Catalytic - genetics
DNA, Catalytic - metabolism
Kinetics
Nucleic Acid Conformation
RNA - genetics
RNA - metabolism
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:We develop a novel functional biosensor on a deoxyribozyme. A 5'-end-immobilized short Ca(2+)-dependent deoxyribozyme (dCGCTGGCAGGCTACAACGAGTCTTC) binds to a target RNA substrate (rGAAGACA decrease UGCCAGCG; decrease denotes an RNA cleavage site), and acts as an enzyme in the presence of Ca2+. It cleaves the target RNA substrate at one site of rAp decrease U in the asymmetric internal loop.
ISSN:0261-3166