Arabidopsis peroxin 16 trafficks through the ER and an intermediate compartment to pre-existing peroxisomes via overlapping molecular targeting signals

Previously it has been shown that the endogenous Arabidopsis peroxin, AtPEX16, coexisted at steady state in membranes of the endoplasmic reticulum (ER) and peroxisomes. Herein, an ER-to-peroxisome trafficking pathway and the requisite molecular targeting signals for mycAtPEX16 transiently expressed...

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Veröffentlicht in:	Journal of experimental botany 2007-01, Vol.58 (7), p.1677-1693
Hauptverfasser:	Karnik, Sheetal K, Trelease, Richard N
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino acids Antibodies Arabidopsis - metabolism Arabidopsis Proteins - chemistry Arabidopsis Proteins - metabolism Arabidopsis thaliana suspension cells ascorbate peroxidase Biological and medical sciences brefeldin A Cell nucleus Cold Temperature Cytosol Endoplasmic reticulum Endoplasmic Reticulum - metabolism Fluorescent antibody techniques Fundamental and applied biological sciences. Psychology Genes. Genome Green Fluorescent Proteins - analysis immunofluorescence microscopy inter-organellar targeting signals Membrane proteins Microscopy Molecular and cellular biology Molecular genetics Peroxins peroxisome membrane protein Peroxisomes Peroxisomes - metabolism Plant cells Protein Sorting Signals Protein Structure, Tertiary Protein Transport Recombinant Fusion Proteins - analysis Research Papers reticular endoplasmic reticulum Sequence Analysis, Protein tobacco BY-2 cells transient protein expression
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Zusammenfassung:	Previously it has been shown that the endogenous Arabidopsis peroxin, AtPEX16, coexisted at steady state in membranes of the endoplasmic reticulum (ER) and peroxisomes. Herein, an ER-to-peroxisome trafficking pathway and the requisite molecular targeting signals for mycAtPEX16 transiently expressed in Arabidopsis and tobacco BY-2 suspension cells are described. Immunofluorescent mycAtPEX16 was observed initially in the cytosol (
ISSN:	0022-0957 1460-2431
DOI:	10.1093/jxb/erm018