In vivo evaluation of 2'-deoxy-2'-[(18)F]fluoro-5-iodo-1-beta-D-arabinofuranosyluracil ([18F]FIAU) and 2'-deoxy-2'-[18F]fluoro-5-ethyl-1-beta-D-arabinofuranosyluracil ([18F]FEAU) as markers for suicide gene expression

FIAU and FEAU were evaluated in vitro and in vivo as markers for HSV1-tk gene expression. In vitro and biodistribution studies were performed in wild type and transduced HT-29 cells using [14C]FIAU and [3H]FEAU. PET imaging was performed using [18F]FIAU and [18F]FEAU. In vitro uptake of [14C]FIAU in...

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Veröffentlicht in:	European journal of nuclear medicine and molecular imaging 2007-06, Vol.34 (6), p.822-829
Hauptverfasser:	Alauddin, Mian M, Shahinian, Antranic, Park, Ryan, Tohme, Michel, Fissekis, John D, Conti, Peter S
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Arabinofuranosyluracil - pharmacology Carbon Radioisotopes DNA - metabolism Fluorine isotopes Fluorine Radioisotopes Gene expression Gene Transfer Techniques Genes, Reporter Genetic Techniques Genetic Therapy - instrumentation Genetic Therapy - methods Humans In vitro methods and tests In vivo methods and tests Kinases Markers Mice Mice, Nude Positron emission Positron-Emission Tomography - instrumentation Positron-Emission Tomography - methods Radiopharmaceuticals - pharmacology Suicide Suicide genes Thymidine Kinase - metabolism TK gene Tomography Tumors
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Zusammenfassung:	FIAU and FEAU were evaluated in vitro and in vivo as markers for HSV1-tk gene expression. In vitro and biodistribution studies were performed in wild type and transduced HT-29 cells using [14C]FIAU and [3H]FEAU. PET imaging was performed using [18F]FIAU and [18F]FEAU. In vitro uptake of [14C]FIAU in tk-positive cells was 39-fold, 49-fold, and 43-fold higher (p
ISSN:	1619-7070 1619-7089
DOI:	10.1007/s00259-006-0305-1