Toxoplasma gondii: DNA vaccination with genes encoding antigens MIC2, M2AP, AMA1 and BAG1 and evaluation of their immunogenic potential
A combination of antigenic regions of microneme proteins have been previously reported as being protective against chronic toxoplasmosis. In this work, we evaluated immune responses induced by immunizing BALB/c and C57BL/6 mice intradermally with plasmid DNA encoding the protein sequences of Toxopla...
Gespeichert in:
Veröffentlicht in: | Experimental parasitology 2007-07, Vol.116 (3), p.273-282 |
---|---|
Hauptverfasser: | , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | A combination of antigenic regions of microneme proteins have been previously reported as being protective against chronic toxoplasmosis. In this work, we evaluated immune responses induced by immunizing BALB/c and C57BL/6 mice intradermally with plasmid DNA encoding the protein sequences of
Toxoplasma gondii AMA1, MIC2, M2AP and BAG1. Mice immunized with the
AMA1 gene developed high levels of serum IgG2a and c antibodies as well as cellular immune responses associated with IFN-γ synthesis suggesting a modulated Th1 type of response. Immunization with the
AMA1 gene resulted in a partial but significant protection against the acute phase of toxoplasmosis compared to
MIC2,
M2AP and
BAG1 genes. Therefore, the
AMA1 gene appears to generate a strong specific immune response and also provides effective protection against toxoplasmosis more than the
MIC2,
M2AP and
BAG1 genes. |
---|---|
ISSN: | 0014-4894 1090-2449 |
DOI: | 10.1016/j.exppara.2007.01.017 |