Dissociation of force decline from calcium decline by preload in isolated rabbit myocardium

It is well known that the rate of intracellular calcium ([Ca 2+ ] i ) decline is an important factor governing relaxation in unloaded myocardium. However, it remains unclear to what extent, under near physiological conditions, the intracellular calcium transient amplitude and kinetics contribute to the length-dependent increase in force and increase in duration of relaxation. We hypothesize that myofilament properties rather than calcium transient decline primarily determines the duration of relaxation in adult mammalian myocardium. To test this hypothesis, we simultaneously measured force of contraction and calibrated [Ca 2+ ] i transients in isolated, thin rabbit trabeculae at various lengths at 37°C. Time from peak tension to 50% relaxation (RT 50(tension) ) increases significantly with length (from 49.8 ± 3.4 to 83.8 ± 7.4 ms at an [Ca 2+ ] o of 2.5 mM), whereas time from peak calcium to 50% decline (RT 50(calcium) ) was not prolonged (from 124.8 ± 5.3 to 107.7 ± 11.4 ms at an [Ca 2+ ] o of 2.5 mM). Analysis of variance revealed that RT 50(tension) is significantly correlated with length ( P