A nasal vaccine comprising B-subunit derivative of Shiga toxin 2 for cross-protection against Shiga toxin types 1 and 2

Summary Enterohemorrhagic Escherichia coli (EHEC) produces Stx1 and Stx2 causing severe diseases. Their B-subunits (StxBs) exhibit low immunogenicity and the anti-StxB antibodies neutralizing both Stxs has not been prepared yet. By intranasal vaccination with His-tagged-StxB (Stx1B-His or Stx2B-His)...

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Veröffentlicht in:	Vaccine 2008-04, Vol.26 (17), p.2092-2099
Hauptverfasser:	Tsuji, Takao, Shimizu, Takeshi, Sasaki, Keiko, Tsukamoto, Kentaro, Arimitsu, Hideyuki, Ochi, Sadayuki, Shimizu, Toshiyasu, Taniguchi, Koki, Noda, Masatoshi, Neri, Paola, Mori, Hiroshi
Format:	Artikel
Sprache:	eng
Schlagworte:	Administration, Intranasal Allergy and Immunology Animals Antibodies, Bacterial - blood Antibodies, Bacterial - immunology Applied microbiology Bacterial Vaccines - administration & dosage Bacterial Vaccines - immunology Bacteriology Biological and medical sciences E coli Enterohemorrhagic Escherichia coli Escherichia coli Escherichia coli Infections - immunology Escherichia coli Infections - prevention & control Escherichia coli Vaccines - immunology Fundamental and applied biological sciences. Psychology Genes Heat-labile enterotoxin Immunization Immunogenicity Laboratory animals Lung - immunology Mice Microbiology Miscellaneous Mucosal adjuvant Nasal vaccine Proteins Shiga Toxin 1 - immunology Shiga Toxin 2 - immunology Stx2B Toxicity Toxins Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Vaccines, Subunit - administration & dosage Vaccines, Subunit - immunology
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Beschreibung
Zusammenfassung:	Summary Enterohemorrhagic Escherichia coli (EHEC) produces Stx1 and Stx2 causing severe diseases. Their B-subunits (StxBs) exhibit low immunogenicity and the anti-StxB antibodies neutralizing both Stxs has not been prepared yet. By intranasal vaccination with His-tagged-StxB (Stx1B-His or Stx2B-His) plus a mutant heat-labile enterotoxin (mLT) in mice, their serum and lung fluid reacted with appropriate StxB. Mice vaccinated with Stx2B-His plus mLT had antibodies reacting Stx1B and showed the resistance to toxemia of Stx1 and Stx2. This is the first demonstration to get anti-Stx2B serum neutralizing both Stxs. These suggest that the nasal vaccination with Stx2B-His and mLT is effective for preventing toxemias by EHEC.
ISSN:	0264-410X 1873-2518
DOI:	10.1016/j.vaccine.2008.02.034