A Gold-Nanoparticle-Based Real-Time Colorimetric Screening Method for Endonuclease Activity and Inhibition

A Gold-Nanoparticle-Based Real-Time Colorimetric Screening Method for Endonuclease Activity and Inhibition

On the small screen: Aggregates of gold nanoparticles interconnected by DNA duplexes (see scheme, left) are bluish‐purple. Cleavage of the duplexes by deoxyribonuclease I (DNase I) releases the nanoparticles (right), producing a bluish‐purple‐to‐red color change. This color change can be used to mon...

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Veröffentlicht in:Angewandte Chemie International Edition 2007-01, Vol.46 (19), p.3468-3470
Hauptverfasser: Xu, Xiaoyang, Han, Min Su, Mirkin, Chad A.
Format: Artikel
Sprache:eng
Schlagworte:
Base Sequence
Biosensing Techniques
endonucleases
Endonucleases - analysis
Endonucleases - antagonists & inhibitors
Endonucleases - metabolism
Gold - chemistry
high‒throughput screening
inhibitors
nanoparticles
Nanoparticles - chemistry
Oligonucleotide Probes - chemistry
oligonucleotides
Spectrophotometry, Ultraviolet
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Beschreibung
Zusammenfassung:On the small screen: Aggregates of gold nanoparticles interconnected by DNA duplexes (see scheme, left) are bluish‐purple. Cleavage of the duplexes by deoxyribonuclease I (DNase I) releases the nanoparticles (right), producing a bluish‐purple‐to‐red color change. This color change can be used to monitor the activity (and inhibition) of the enzyme.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.200605249