Flavobacterium anhuiense sp. nov., isolated from field soil

1 Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China 2 Biotoxin Department of Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, PR China Correspondence Ren Lai rlai72{at}njau.edu.cn A novel strain, D3 T , isolated from a field-soil sample obtained from Anhui Province, PR China, was characterized taxonomically by using a polyphasic approach. The cells were Gram-negative, yellow-pigmented rods devoid of flagella, but showing gliding motility. The organism was able to grow at 5–37 °C and at pH 4.0–10.0. A comparative 16S rRNA gene sequence analysis indicated that strain D3 T is a member of the genus Flavobacterium , sharing highest sequence similarity with the type strain of Flavobacterium defluvii (96.7 %). The major isoprenoid quinone was MK-6 and the predominant fatty acids were iso-C 15 : 0 , summed feature 3 (C 16 : 1 7 c and/or iso-C 15 : 0 2-OH) and C 16 : 0 . The DNA G+C content was 31.4 mol%. On the basis of phylogenetic and phenotypic data, strain D3 T represents a novel species within the genus Flavobacterium , for which the name Flavobacterium anhuiense sp. nov. is proposed. The type strain is D3 T (=KCTC 22128 T =CGMCC 1.6859 T ). These authors contributed equally to this paper. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain D3 T is EU046269 . Transmission electron micrographs of strain D3 T are available with the online version of this paper.