Online Preconcentration by Transient Isotachophoresis in Linear Polymer on a Poly(methyl methacrylate) Microchip for Separation of Human Serum Albumin Immunoassay Mixtures

Online preconcentration of human serum albumin (HSA) and its immunocomplex with a monoclonal antibody by on-chip transient isotachophoresis is reported. An 800-fold signal enhancement was achieved following the preconcentration on standard cross-channel microchips made of poly (methyl methacrylate)....

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Veröffentlicht in:	Analytical chemistry (Washington) 2007-05, Vol.79 (10), p.3667-3672
Hauptverfasser:	Mohamadi, Mohamad Reza, Kaji, Noritada, Tokeshi, Manabu, Baba, Yoshinobu
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Antibodies, Monoclonal Antigen-Antibody Complex - isolation & purification Blood Chemical compounds Chemistry Chromatographic methods and physical methods associated with chromatography Electrophoresis, Microchip - instrumentation Electrophoresis, Microchip - methods Exact sciences and technology Humans Immunoassay Immunoassay - methods Miscellaneous Other chromatographic methods Polymers Polymethyl Methacrylate Proteins Serum Albumin - immunology Serum Albumin - isolation & purification Spectrometric and optical methods
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