In situ detection of programmed cell death in Nicotiana tabacum leaves during senescence

In situ detection of programmed cell death in Nicotiana tabacum leaves during senescence

Leaf senescence is a highly regulated developmental process, during which cell constituents are dismantled in an ordered progression. The final death phase of senescence is a type of programmed cell death (PCD). We showed DNA fragmentation characteristic for PCD by terminal deoxynucleotidyl transfer...

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Veröffentlicht in:Journal of microscopy (Oxford) 2008-04, Vol.230 (1), p.1-3
Hauptverfasser: UZELAC, B, JANOŠEVIĆ, D, BUDIMIR, S
Format: Artikel
Sprache:eng
Schlagworte:
Aging
Apoptosis
DNA Fragmentation
DNA, Plant - metabolism
In Situ Nick-End Labeling
leaf senescence
Microscopy, Fluorescence
Nicotiana - physiology
Nicotiana tabacum
Plant Leaves - physiology
programmed cell death
Staining and Labeling - methods
TUNEL assay
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Beschreibung
Zusammenfassung:Leaf senescence is a highly regulated developmental process, during which cell constituents are dismantled in an ordered progression. The final death phase of senescence is a type of programmed cell death (PCD). We showed DNA fragmentation characteristic for PCD by terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL) assay not only in senescing leaves but also in young, still developing tobacco leaves.
ISSN:0022-2720
1365-2818
DOI:10.1111/j.1365-2818.2008.01947.x