Influence of culture conditions on mycelial growth and bioluminescence of Gerronema viridilucens

Abstract Herein we describe a procedure for measuring the total light emission of the naturally bioluminescent tropical fungus Gerronema viridilucens and the optimization of culture conditions using multivariate factorial anova. Cultures growing on an agar surface in 35 mm Petri dishes at 90% humidi...

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Veröffentlicht in:	FEMS microbiology letters 2008-05, Vol.282 (1), p.132-139
Hauptverfasser:	Mendes, Luiz F., Bastos, Erick L., Desjardin, Dennis E., Stevani, Cassius V.
Format:	Artikel
Sprache:	eng
Schlagworte:	Agaricales - growth & development Agaricales - metabolism Bacterial Proteins - metabolism Biological and medical sciences Bioluminescence Biomass Brazil Culture Culture Media - chemistry ecotoxicology Emission measurements experimental design Fundamental and applied biological sciences. Psychology fungal physiology Fungi Hydrogen-Ion Concentration Light emission Luminescent Proteins - metabolism Microbiology Molasses Mycelia Mycelium - growth & development Mycelium - metabolism Optimization pH effects Sugar Sugarcane Temperature Yeast Yeasts
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Zusammenfassung:	Abstract Herein we describe a procedure for measuring the total light emission of the naturally bioluminescent tropical fungus Gerronema viridilucens and the optimization of culture conditions using multivariate factorial anova. Cultures growing on an agar surface in 35 mm Petri dishes at 90% humidity show optimal bioluminescence emission at 25 °C in the presence of 1.0% sugar cane molasses, 0.10% yeast extract and pH 6.0 (nonbuffered). Temperature and pH are the most important factors for both mycelial growth and bioluminescence.
ISSN:	0378-1097 1574-6968
DOI:	10.1111/j.1574-6968.2008.01118.x