Comparative sequence analysis of 16S rRNA gene of Pasteurella multocida serogroup B isolates from different animal species

The phylogenetic relationships of five isolates of Pasteurella multocida serotype B:2 belonging to buffalo, cattle, pig, sheep and goat were investigated by comparative sequence analysis of 16S rRNA gene. The 1468 bp fragment of 16S rRNA gene sequence comparison showed that the isolates of cattle (P...

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Veröffentlicht in:	Research in veterinary science 2007-08, Vol.83 (1), p.1-4
Hauptverfasser:	Dey, S., Singh, V.P., Kumar, A.A., Sharma, B., Srivastava, S.K., Singh, Nem
Format:	Artikel
Sprache:	eng
Schlagworte:	16S rRNA Animal diseases Animals Bacterial Vaccines - immunology Base Sequence buffaloes Buffaloes - microbiology Cattle Deoxyribonucleic acid DNA Epidemiology Gene amplification Genes, Bacterial - genetics genetic distance genetic polymorphism genetic variation Genetics geographical variation Goats Goats - microbiology Haemorrhagic septicaemia Hemorrhagic Septicemia - microbiology Hemorrhagic Septicemia - prevention & control Hemorrhagic Septicemia - veterinary Hogs microbial genetics Microbiology molecular epidemiology molecular sequence data Molecular weight nucleotide sequences Pasteurella multocida Pasteurella multocida - classification Pasteurella multocida - genetics Pasteurella multocida - isolation & purification pasteurellosis Phylogenetics Phylogeny polymerase chain reaction ribosomal RNA RNA, Ribosomal, 16S - genetics septicemia Serotype serotypes Sheep Sheep - microbiology strains swine Swine - microbiology Veterinary medicine
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Zusammenfassung:	The phylogenetic relationships of five isolates of Pasteurella multocida serotype B:2 belonging to buffalo, cattle, pig, sheep and goat were investigated by comparative sequence analysis of 16S rRNA gene. The 1468 bp fragment of 16S rRNA gene sequence comparison showed that the isolates of cattle (PM75), pig (PM49) and sheep (PM82) shared 99.9% homology with the buffalo isolate (vaccine strain P 52) whereas, the goat isolate (PM86) shared 99.8% homology with the vaccine strain. The 16S rRNA gene sequences of these isolates were also found monophyletic with type B reference strain NCTC 10323 of P. multocida subsp. multocida. The present study indicated the close relationships of haemorrhagic septicaemia causing P. multocida serotype B:2 isolates of buffalo and cattle with other uncommon hosts (pig, sheep and goat).
ISSN:	0034-5288 1532-2661
DOI:	10.1016/j.rvsc.2006.10.002