A Near-Infrared-Fluorescence-Quenched Gold-Nanoparticle Imaging Probe for In Vivo Drug Screening and Protease Activity Determination

A Near-Infrared-Fluorescence-Quenched Gold-Nanoparticle Imaging Probe for In Vivo Drug Screening and Protease Activity Determination

Nanodiagnosis: A matrix‐metalloproteinase (MMP) sensitive gold‐nanoparticle (AuNP) imaging probe quenches conjugated near‐infrared (NIR) dyes with high efficiency and is specifically activated by the target MMPs (see picture, left). With this system, nanomolar amounts of protease can be detected—bot...

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Veröffentlicht in:Angewandte Chemie International Edition 2008-03, Vol.47 (15), p.2804-2807
Hauptverfasser: Lee, Seulki, Cha, Eui-Joon, Park, Kyeongsoon, Lee, Seung-Young, Hong, Jin-Ki, Sun, In-Cheol, Kim, Sang Yoon, Choi, Kuiwon, Kwon, Ick Chan, Kim, Kwangmeyung, Ahn, Cheol-Hee
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Antineoplastic Agents - analysis
Antineoplastic Agents - chemistry
Antineoplastic Agents - pharmacology
cancer diagnosis
Cell Line, Tumor
drug screening
Drug Screening Assays, Antitumor - instrumentation
Drug Screening Assays, Antitumor - methods
fluorescent probes
gold
Gold - chemistry
Humans
imaging agents
Metal Nanoparticles - chemistry
Metal Nanoparticles - ultrastructure
Mice
Mice, Nude
Microscopy, Electron, Transmission
Peptide Hydrolases - analysis
Peptide Hydrolases - metabolism
Protease Inhibitors - analysis
Protease Inhibitors - chemistry
Protease Inhibitors - pharmacology
Spectrometry, Fluorescence
Spectrophotometry, Infrared
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Beschreibung
Zusammenfassung:Nanodiagnosis: A matrix‐metalloproteinase (MMP) sensitive gold‐nanoparticle (AuNP) imaging probe quenches conjugated near‐infrared (NIR) dyes with high efficiency and is specifically activated by the target MMPs (see picture, left). With this system, nanomolar amounts of protease can be detected—both in vitro and in vivo. Experiments disclose an apparent positive contrast in MMPs‐positive tumor‐bearing mice (right).
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.200705240