Preparative-Scale Chromatography of Ecdysteroids: A Class of Biologically Active Steroids
A simple separation procedure is developed for the isolation of the main phytoecdysteroid 20-hydroxyecdysone from the herb Silene viridiflora. The purification in four steps uses only a simple preparative-scale separation technique (i.e., liquid-liquid extraction, precipitation, solid-phase extracti...
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| Veröffentlicht in: | Journal of chromatographic science 2008-02, Vol.46 (2), p.111-116 |
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| container_title | Journal of chromatographic science |
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| creator | Toth, Noemi Bathori, Maria |
| description | A simple separation procedure is developed for the isolation of the main phytoecdysteroid 20-hydroxyecdysone from the herb Silene viridiflora. The purification in four steps uses only a simple preparative-scale separation technique (i.e., liquid-liquid extraction, precipitation, solid-phase extraction on octadecyl silica, and crystallization). This procedure is extended using classical normal-phase liquid column chromatography, rotation planar chromatography, and preparative high-performance liquid chromatography for the isolation of the minor ecdysteroids: integristerone A, 26-hydroxypolypodine B, 2-deoxy-20,26- dihydroxyecdysone, and polypodine B. 2-Deoxy-20,26- dihydroxyecdysone is isolated from this species for the first time. The isolation of these ecdysteroids in adequate amounts makes them readily available for insect physiology experiments and for structure-activity relationship studies. The preparative-scale separation work also results in a minor, as yet unknown ecdysteroid. |
| doi_str_mv | 10.1093/chromsci/46.2.111 |
| format | Article |
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The purification in four steps uses only a simple preparative-scale separation technique (i.e., liquid-liquid extraction, precipitation, solid-phase extraction on octadecyl silica, and crystallization). This procedure is extended using classical normal-phase liquid column chromatography, rotation planar chromatography, and preparative high-performance liquid chromatography for the isolation of the minor ecdysteroids: integristerone A, 26-hydroxypolypodine B, 2-deoxy-20,26- dihydroxyecdysone, and polypodine B. 2-Deoxy-20,26- dihydroxyecdysone is isolated from this species for the first time. The isolation of these ecdysteroids in adequate amounts makes them readily available for insect physiology experiments and for structure-activity relationship studies. The preparative-scale separation work also results in a minor, as yet unknown ecdysteroid.</description><identifier>ISSN: 0021-9665</identifier><identifier>EISSN: 1945-239X</identifier><identifier>DOI: 10.1093/chromsci/46.2.111</identifier><identifier>PMID: 18366868</identifier><language>eng</language><publisher>United States: Oxford University Press</publisher><subject>Chromatography, Liquid - methods ; Ecdysteroids - analysis ; Ecdysteroids - chemistry ; Ecdysteroids - isolation & purification ; Ecdysterone - analysis ; Ecdysterone - chemistry ; Ecdysterone - isolation & purification ; Mass Spectrometry ; Nuclear Magnetic Resonance, Biomolecular ; Silene - chemistry</subject><ispartof>Journal of chromatographic science, 2008-02, Vol.46 (2), p.111-116</ispartof><rights>2008</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-4995838819fdc4ee2816dc14a864dbeb04a4ef1c58a4d9460aeab8e632a6003d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1578,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18366868$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Toth, Noemi</creatorcontrib><creatorcontrib>Bathori, Maria</creatorcontrib><title>Preparative-Scale Chromatography of Ecdysteroids: A Class of Biologically Active Steroids</title><title>Journal of chromatographic science</title><addtitle>Journal of Chromatographic Science</addtitle><addtitle>Journal of Chromatographic Science</addtitle><description>A simple separation procedure is developed for the isolation of the main phytoecdysteroid 20-hydroxyecdysone from the herb Silene viridiflora. 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The preparative-scale separation work also results in a minor, as yet unknown ecdysteroid.</description><subject>Chromatography, Liquid - methods</subject><subject>Ecdysteroids - analysis</subject><subject>Ecdysteroids - chemistry</subject><subject>Ecdysteroids - isolation & purification</subject><subject>Ecdysterone - analysis</subject><subject>Ecdysterone - chemistry</subject><subject>Ecdysterone - isolation & purification</subject><subject>Mass Spectrometry</subject><subject>Nuclear Magnetic Resonance, Biomolecular</subject><subject>Silene - chemistry</subject><issn>0021-9665</issn><issn>1945-239X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkEFPwjAcRxujEUQ_gBezkycH7dqV1htMFBMSSdCIXpqu63A67Gw34769W4Z69dT80_d7hwfAKYJDBDkeqRdrtk5lI0KHwRAhtAf6iJPQDzBf74M-hAHyOaVhDxw599qeiIWHoIcYppRR1gdPS6sLaWWZfWp_pWSuvai1ytJsrCxeas-k3kwltSu1NVniLr2JF-XSufZjmpncbLJmltfeRLUSb7UDj8FBKnOnT3bvADxcz-6jub-4u7mNJgtfETQufcJ5yDBjiKeJIloHDNFEISIZJUmsY0gk0SlSIZMk4YRCqWXMNMWBpBDiBA_AeectrPmotCvFNnNK57l816ZyYgwJJg3ZgKgDlTXOWZ2KwmZbaWuBoGh7ip-eglARiKZnsznbyat4q5O_xS5gA1x0gKmKf_n8Ds-anl-_A2nfBB3jcSjm62exfryaLvmKiQh_A2Fhkiw</recordid><startdate>20080201</startdate><enddate>20080201</enddate><creator>Toth, Noemi</creator><creator>Bathori, Maria</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20080201</creationdate><title>Preparative-Scale Chromatography of Ecdysteroids: A Class of Biologically Active Steroids</title><author>Toth, Noemi ; Bathori, Maria</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-4995838819fdc4ee2816dc14a864dbeb04a4ef1c58a4d9460aeab8e632a6003d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Chromatography, Liquid - methods</topic><topic>Ecdysteroids - analysis</topic><topic>Ecdysteroids - chemistry</topic><topic>Ecdysteroids - isolation & purification</topic><topic>Ecdysterone - analysis</topic><topic>Ecdysterone - chemistry</topic><topic>Ecdysterone - isolation & purification</topic><topic>Mass Spectrometry</topic><topic>Nuclear Magnetic Resonance, Biomolecular</topic><topic>Silene - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Toth, Noemi</creatorcontrib><creatorcontrib>Bathori, Maria</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of chromatographic science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Toth, Noemi</au><au>Bathori, Maria</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparative-Scale Chromatography of Ecdysteroids: A Class of Biologically Active Steroids</atitle><jtitle>Journal of chromatographic science</jtitle><stitle>Journal of Chromatographic Science</stitle><addtitle>Journal of Chromatographic Science</addtitle><date>2008-02-01</date><risdate>2008</risdate><volume>46</volume><issue>2</issue><spage>111</spage><epage>116</epage><pages>111-116</pages><issn>0021-9665</issn><eissn>1945-239X</eissn><abstract>A simple separation procedure is developed for the isolation of the main phytoecdysteroid 20-hydroxyecdysone from the herb Silene viridiflora. The purification in four steps uses only a simple preparative-scale separation technique (i.e., liquid-liquid extraction, precipitation, solid-phase extraction on octadecyl silica, and crystallization). This procedure is extended using classical normal-phase liquid column chromatography, rotation planar chromatography, and preparative high-performance liquid chromatography for the isolation of the minor ecdysteroids: integristerone A, 26-hydroxypolypodine B, 2-deoxy-20,26- dihydroxyecdysone, and polypodine B. 2-Deoxy-20,26- dihydroxyecdysone is isolated from this species for the first time. The isolation of these ecdysteroids in adequate amounts makes them readily available for insect physiology experiments and for structure-activity relationship studies. 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| ispartof | Journal of chromatographic science, 2008-02, Vol.46 (2), p.111-116 |
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| language | eng |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free Full-Text Journals in Chemistry |
| subjects | Chromatography, Liquid - methods Ecdysteroids - analysis Ecdysteroids - chemistry Ecdysteroids - isolation & purification Ecdysterone - analysis Ecdysterone - chemistry Ecdysterone - isolation & purification Mass Spectrometry Nuclear Magnetic Resonance, Biomolecular Silene - chemistry |
| title | Preparative-Scale Chromatography of Ecdysteroids: A Class of Biologically Active Steroids |
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