Preparative-Scale Chromatography of Ecdysteroids: A Class of Biologically Active Steroids
A simple separation procedure is developed for the isolation of the main phytoecdysteroid 20-hydroxyecdysone from the herb Silene viridiflora. The purification in four steps uses only a simple preparative-scale separation technique (i.e., liquid-liquid extraction, precipitation, solid-phase extracti...
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Veröffentlicht in: | Journal of chromatographic science 2008-02, Vol.46 (2), p.111-116 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | A simple separation procedure is developed for the isolation of the main phytoecdysteroid 20-hydroxyecdysone from the herb Silene viridiflora. The purification in four steps uses only a simple preparative-scale separation technique (i.e., liquid-liquid extraction, precipitation, solid-phase extraction on octadecyl silica, and crystallization). This procedure is extended using classical normal-phase liquid column chromatography, rotation planar chromatography, and preparative high-performance liquid chromatography for the isolation of the minor ecdysteroids: integristerone A, 26-hydroxypolypodine B, 2-deoxy-20,26- dihydroxyecdysone, and polypodine B. 2-Deoxy-20,26- dihydroxyecdysone is isolated from this species for the first time. The isolation of these ecdysteroids in adequate amounts makes them readily available for insect physiology experiments and for structure-activity relationship studies. The preparative-scale separation work also results in a minor, as yet unknown ecdysteroid. |
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ISSN: | 0021-9665 1945-239X |
DOI: | 10.1093/chromsci/46.2.111 |