Examining Ca2+ Extrusion of Na+/Ca2+-K+ Exchangers

Examining Ca2+ Extrusion of Na+/Ca2+-K+ Exchangers

:  Na+/Ca2+‐K+ exchangers (NCKX) are plasma membrane transporters that are thought to mainly mediate Ca2+ extrusion (along with K+) at the expense of the Na+ electrochemical gradient. However, because they are bidirectional, most assays have relied on measuring their activity in the reverse (Ca2+ im...

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Veröffentlicht in:Annals of the New York Academy of Sciences 2007-03, Vol.1099 (1), p.29-33
Hauptverfasser: ALTIMIMI, HAIDER F., SCHNETKAMP, PAUL P. M.
Format: Artikel
Sprache:eng
Schlagworte:
Calcium - metabolism
calcium extrusion
calcium sequestration
Cell Line
Electrochemistry
Humans
Na+/Ca2+-K+ exchangers
NCKX
Sodium-Calcium Exchanger - metabolism
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Zusammenfassung::  Na+/Ca2+‐K+ exchangers (NCKX) are plasma membrane transporters that are thought to mainly mediate Ca2+ extrusion (along with K+) at the expense of the Na+ electrochemical gradient. However, because they are bidirectional, most assays have relied on measuring their activity in the reverse (Ca2+ import) mode. Herein we describe a method to control intracellular ionic conditions, and examine the forward (Ca2+ extrusion) mode of exchange of NCKX2.
ISSN:0077-8923
1749-6632
DOI:10.1196/annals.1387.056