Gold Nanohole Array Substrates as Immunobiosensors

A gold nanohole array is functionalized with a cortisol thiol derivative, and binding to a monoclonal antibody is conveniently detected using the sensitive shift in the 1060 nm transmission peak of the array. Detection is also enhanced 3-fold by the application of a secondary antibody−gold nanoparti...

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Veröffentlicht in:	Analytical chemistry (Washington) 2008-03, Vol.80 (6), p.2244-2249
Hauptverfasser:	Sharpe, John C, Mitchell, John S, Lin, Ling, Sedoglavich, Nemanya, Blaikie, Richard J
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Antibodies, Monoclonal - chemistry Biological and medical sciences Biosensing Techniques Biosensors Biotechnology Chemical compounds Fundamental and applied biological sciences. Psychology Gold Gold - chemistry Hydrocortisone - chemistry Immunoassay - instrumentation Methods. Procedures. Technologies Molecules Monoclonal antibodies Nanoparticles Nanostructures Various methods and equipments
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Beschreibung
Zusammenfassung:	A gold nanohole array is functionalized with a cortisol thiol derivative, and binding to a monoclonal antibody is conveniently detected using the sensitive shift in the 1060 nm transmission peak of the array. Detection is also enhanced 3-fold by the application of a secondary antibody−gold nanoparticle conjugate. This regenerable response represents a more sensitive shift than that obtained previously for higher affinity binding and opens the way to application of nanohole arrays in immunobiosensing of important biomolecules.
ISSN:	0003-2700 1520-6882
DOI:	10.1021/ac702555r