An approach to assay calcineurin activity and the inhibitory effect of zinc ion

In this study, an electrochemical method to assay calcineurin activity is proposed. Although the enzyme could not exhibit electron transfer reactivity and the catalytic reaction of the substrate could not give any electrochemical wave, p-nitrophenol as the catalytic reaction production could be oxidized at the calcineurin/Triton X-100 film modified electrode to exhibit useful wave that might be employed to assay the enzyme activity. The effect of Ni2+ and Zn2+ on calcineurin was also investigated. Whereas Ni2+ was confirmed to be able to enhance the enzymatic activity, Zn2+ was found to be an inhibitor to calcineurin.