Evolving a Thermostable DNA Polymerase That Amplifies from Highly Damaged Templates

The foreign lesion: DNAs with damage generated, for example, by UV light are not good targets for standard replicative DNA polymerases. A DNA polymerase has been derived by directed evolution that faithfully amplifies light‐damaged DNA. Interestingly, a single mutation is sufficient for this propert...

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Veröffentlicht in:	Angewandte Chemie International Edition 2007-01, Vol.46 (17), p.3115-3117
Hauptverfasser:	Gloeckner, Christian, Sauter, Katharina B. M., Marx, Andreas
Format:	Artikel
Sprache:	eng
Schlagworte:	directed evolution Directed Molecular Evolution DNA - biosynthesis DNA - chemistry DNA - genetics DNA - metabolism DNA Damage DNA polymerase DNA Replication DNA-Directed DNA Polymerase - genetics DNA-Directed DNA Polymerase - metabolism Enzyme Stability Gene Amplification Molecular Structure polymerase chain reaction Temperature Templates, Genetic Thermodynamics
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Beschreibung
Zusammenfassung:	The foreign lesion: DNAs with damage generated, for example, by UV light are not good targets for standard replicative DNA polymerases. A DNA polymerase has been derived by directed evolution that faithfully amplifies light‐damaged DNA. Interestingly, a single mutation is sufficient for this property (see picture).
ISSN:	1433-7851 1521-3773
DOI:	10.1002/anie.200603987