A combined immunofluorescence and fluorescent in situ hybridization assay for single cell analyses of dental plaque microorganisms

A IF–FISH protocol was developed to characterize discrete cell populations within complex dental plaque samples. Optimizing and shortening the 3-step IF procedure and including RNase inhibitor in all IF reagents enabled the combination of both techniques and thus the simultaneous detection and enume...

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Veröffentlicht in:	Journal of microbiological methods 2007-05, Vol.69 (2), p.402-405
Hauptverfasser:	Gmür, Rudolf, Lüthi-Schaller, Helga
Format:	Artikel
Sprache:	eng
Schlagworte:	16S rRNA Bacteria - genetics Bacteria - isolation & purification Bacteriological methods and techniques used in bacteriology Bacteriology Biofilm Biological and medical sciences Dental plaque Dental Plaque - microbiology Fluorescent Antibody Technique, Direct - methods Fluorescent in situ hybridization Fundamental and applied biological sciences. Psychology Humans Immunofluorescence In Situ Hybridization, Fluorescence - methods Microbiology Microscopy, Fluorescence Periodontitis - microbiology
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