A combined immunofluorescence and fluorescent in situ hybridization assay for single cell analyses of dental plaque microorganisms

A IF–FISH protocol was developed to characterize discrete cell populations within complex dental plaque samples. Optimizing and shortening the 3-step IF procedure and including RNase inhibitor in all IF reagents enabled the combination of both techniques and thus the simultaneous detection and enume...

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Veröffentlicht in:	Journal of microbiological methods 2007-05, Vol.69 (2), p.402-405
Hauptverfasser:	Gmür, Rudolf, Lüthi-Schaller, Helga
Format:	Artikel
Sprache:	eng
Schlagworte:	16S rRNA Bacteria - genetics Bacteria - isolation & purification Bacteriological methods and techniques used in bacteriology Bacteriology Biofilm Biological and medical sciences Dental plaque Dental Plaque - microbiology Fluorescent Antibody Technique, Direct - methods Fluorescent in situ hybridization Fundamental and applied biological sciences. Psychology Humans Immunofluorescence In Situ Hybridization, Fluorescence - methods Microbiology Microscopy, Fluorescence Periodontitis - microbiology
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Beschreibung
Zusammenfassung:	A IF–FISH protocol was developed to characterize discrete cell populations within complex dental plaque samples. Optimizing and shortening the 3-step IF procedure and including RNase inhibitor in all IF reagents enabled the combination of both techniques and thus the simultaneous detection and enumeration of bacterial populations with distinct 16S rRNA sequences and/or surface markers of interest.
ISSN:	0167-7012 1872-8359
DOI:	10.1016/j.mimet.2006.12.012