Novel Loop-Mediated Isothermal Amplification Method for Detection of the JP2 Clone of Aggregatibacter actinomycetemcomitans in Subgingival Plaque

We developed a loop-mediated isothermal amplification method that detects the JP2 clone of Aggregatibacter actinomycetemcomitans, which induces aggressive periodontitis in adolescents of North and West African descents. Being independent of special equipment, this specific and sensitive method offer...

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Veröffentlicht in:	Journal of Clinical Microbiology 2008-03, Vol.46 (3), p.1113-1115
Hauptverfasser:	Seki, Mitsuko, Poulsen, Knud, Haubek, Dorte, Kilian, Mogens
Format:	Artikel
Sprache:	eng
Schlagworte:	Actinobacillus Infections - microbiology Adolescent Aggregatibacter actinomycetemcomitans - genetics Aggregatibacter actinomycetemcomitans - isolation & purification Aggressive Periodontitis - microbiology Bacterial diseases Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Dental Plaque - microbiology DNA, Bacterial - analysis DNA, Bacterial - isolation & purification Ent and stomatologic bacterial diseases Fundamental and applied biological sciences. Psychology Human bacterial diseases Humans Infectious diseases Medical sciences Microbiology Morocco Nucleic Acid Amplification Techniques - methods Sensitivity and Specificity
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Beschreibung
Zusammenfassung:	We developed a loop-mediated isothermal amplification method that detects the JP2 clone of Aggregatibacter actinomycetemcomitans, which induces aggressive periodontitis in adolescents of North and West African descents. Being independent of special equipment, this specific and sensitive method offers significant advantages for screening of patients on a population basis and in clinical settings.
ISSN:	0095-1137 1098-660X 1098-5530
DOI:	10.1128/JCM.02107-07