Free-Energy Landscape of Enzyme Catalysis

The concept is developed that enzyme mechanisms should be viewed as “catalytic networks” with multiple conformations that occur serially and in parallel in the mechanism. These coupled ensembles of conformations require a multi-dimensional standard free-energy surface that is very “rugged”, containi...

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Veröffentlicht in:	Biochemistry (Easton) 2008-03, Vol.47 (11), p.3317-3321
Hauptverfasser:	Benkovic, Stephen J., Hammes, Gordon G., Hammes-Schiffer, Sharon
Format:	Artikel
Sprache:	eng
Schlagworte:	Catalysis Escherichia coli Proteins - chemistry Escherichia coli Proteins - genetics Escherichia coli Proteins - metabolism Kinetics Protein Conformation Tetrahydrofolate Dehydrogenase - chemistry Tetrahydrofolate Dehydrogenase - genetics Tetrahydrofolate Dehydrogenase - metabolism Thermodynamics
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Beschreibung
Zusammenfassung:	The concept is developed that enzyme mechanisms should be viewed as “catalytic networks” with multiple conformations that occur serially and in parallel in the mechanism. These coupled ensembles of conformations require a multi-dimensional standard free-energy surface that is very “rugged”, containing multiple minima and transition states. Experimental and theoretical evidence is presented to support this concept.
ISSN:	0006-2960 1520-4995
DOI:	10.1021/bi800049z