Effects of KIOM-79 on hyperglycemia and diabetic nephropathy in type 2 diabetic Goto-Kakizaki rats

We investigated the effect of KIOM-79, 80% ethanolic extract of a new herbal prescription, on non-obese type 2 diabetic Goto-Kakizaki (GK) rats. The rats were treated orally with KIOM-79 (500 mg/kg body weight) once a day for 13 weeks to examine the long-term effects on hyperglycemia and glomerular histology as well as biochemical and functional abnormalities in kidney. As the results, we found that KIOM-79 reduced hyperglycemia ( p < 0.01), ameliorated insulin resistance ( p < 0.001), urinary protein excretion ( p < 0.01) and creatinine clearance (Ccr) ( p < 0.001), and inhibited glomerular AGE formation ( p < 0.001) in diabetic GK rats. We also found that KIOM-79 prevented the glomeruli enlargement, overexpression of type IV collagen ( p < 0.001), PKC protein ( p < 0.01), TGF-β mRNA ( p < 0.05) and VEGF mRNA ( p < 0.05). Thus, based on our finding, KIOM-79 could reduce the hyperglycemia, and prevent or retard the development of diabetic nephropathy.