Live Imaging of Neuroinflammation Reveals Sex and Estrogen Effects on Astrocyte Response to Ischemic Injury

We sought to develop a model system for live analysis of brain inflammatory response in ischemic injury. Using a reporter mouse-expressing luciferase gene under transcriptional control of the murine glial fibrillary acidic protein (GFAP) promoter (GFAP-luc mice) and biophotonic/bioluminescent imagin...

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Veröffentlicht in:Stroke (1970) 2008-03, Vol.39 (3), p.935-942
Hauptverfasser: CORDEAU, Pierre, LALANCETTE-HEBERT, Mélanie, YUAN CHENG WENG, KRIZ, Jasna
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Sprache:eng
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Zusammenfassung:We sought to develop a model system for live analysis of brain inflammatory response in ischemic injury. Using a reporter mouse-expressing luciferase gene under transcriptional control of the murine glial fibrillary acidic protein (GFAP) promoter (GFAP-luc mice) and biophotonic/bioluminescent imaging as tools, we developed a model system for in vivo analysis of astrocyte activation/response in cerebral ischemia. Analysis of photon emissions from the brains of living animals revealed marked sex differences in astrocyte response to ischemic injury. The increase in GFAP signals was significantly higher in female mice in the metestrus/diestrus period compared with male transgenic mice (1.71 x 10(7)+/-0.19 x 10(7) vs 0.92 x 10(7)+/-0.15 x 10(7), P
ISSN:0039-2499
1524-4628
DOI:10.1161/STROKEAHA.107.501460