Isolation and oligomeric composition of cytochrome c nitrite reductase from the haloalkaliphilic bacterium Thioalkalivibrio nitratireducens

Isolation and oligomeric composition of cytochrome c nitrite reductase from the haloalkaliphilic bacterium Thioalkalivibrio nitratireducens

A new procedure for isolation of cytochrome c nitrite reductase from the haloalkaliphilic bacterium Thioalkalivibrio nitratireducens increasing significantly the yield of the purified enzyme is presented. The enzyme is isolated from the soluble fraction of the cell extract as a hexamer, as shown by...

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Veröffentlicht in:Biochemistry (Moscow) 2008-02, Vol.73 (2), p.164-170
Hauptverfasser: Tikhonova, T. V, Slutskaya, E. S, Filimonenkov, A. A, Boyko, K. M, Kleimenov, S. Yu, Konarev, P. V, Polyakov, K. M, Svergun, D. I, Trofimov, A. A, Khomenkov, V. G, Zvyagilskaya, R. A, Popov, V. O
Format: Artikel
Sprache:eng
Schlagworte:
Bacterial Proteins - chemistry
Bacterial Proteins - isolation & purification
Biochemistry
Biomedical and Life Sciences
Biomedicine
Bioorganic Chemistry
Chromatography, Gel
Chromatography, Ion Exchange
Cytochromes a1 - chemistry
Cytochromes a1 - isolation & purification
Cytochromes c1 - chemistry
Cytochromes c1 - isolation & purification
Ectothiorhodospiraceae - enzymology
Enzyme Stability
Life Sciences
Microbiology
Nitrate Reductases - chemistry
Nitrate Reductases - isolation & purification
Scattering, Small Angle
Temperature
X-Ray Diffraction
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Beschreibung
Zusammenfassung:A new procedure for isolation of cytochrome c nitrite reductase from the haloalkaliphilic bacterium Thioalkalivibrio nitratireducens increasing significantly the yield of the purified enzyme is presented. The enzyme is isolated from the soluble fraction of the cell extract as a hexamer, as shown by gel filtration chromatography and small angle X-ray scattering analysis. Thermostability of the hexameric form of the nitrite reductase is characterized in terms of thermoinactivation and thermodenaturation.
ISSN:0006-2979
1608-3040
DOI:10.1134/S0006297908020077