Superparamagnetic Iron Oxide Labeling and Transplantation of Adipose-Derived Stem Cells in Middle Cerebral Artery Occlusion-Injured Mice
Adipose-derived stem cells are an alternative stem cell source for CNS therapies. The goals of the current study were to label adipose-derived stem cells with superparamagnetic iron oxide (SPIO) particles, to use MRI to guide the transplantation of adipose-derived stem cells in middle cerebral arter...
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| Veröffentlicht in: | American journal of roentgenology (1976) 2007-04, Vol.188 (4), p.1101-1108 |
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| container_title | American journal of roentgenology (1976) |
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| creator | Rice, Henry E Hsu, Edward W Sheng, Huaxin Evenson, Debra A Freemerman, Alex J Safford, Kristine M Provenzale, James M Warner, David S Johnson, G. Allan |
| description | Adipose-derived stem cells are an alternative stem cell source for CNS therapies. The goals of the current study were to label adipose-derived stem cells with superparamagnetic iron oxide (SPIO) particles, to use MRI to guide the transplantation of adipose-derived stem cells in middle cerebral artery occlusion (MCAO)-injured mice, and to localize donor adipose-derived stem cells in the injured brain using MRI. We hypothesized that we would successfully label adipose-derived stem cells and image them with MRI.
Adipose-derived stem cells harvested from mice inbred for green fluorescent protein were labeled with SPIO ferumoxide particles through the use of poly-L-lysine. Adipose-derived stem cell viability, iron staining, and proliferation were measured after SPIO labeling, and the sensitivity of MRI in the detection of SPIO-labeled adipose-derived stem cells was assessed ex vivo. Adult mice (n = 12) were subjected to unilateral MCAO. Two weeks later, in vivo 7-T MRI was performed to guide stereotactic transplantation of SPIO-labeled adipose-derived stem cells into brain tissue adjacent to the infarct. After 24 hours, the mice were sacrificed for high-resolution ex vivo 7-T or 9.4-T MRI and histologic study.
Adipose-derived stem cells were efficiently labeled with SPIO particles without loss of cell viability or proliferation. Using MRI, we guided precise transplantation of adipose-derived stem cells. MR images of mice given injections of SPIO-labeled adipose-derived stem cells had hypointense regions that correlated with the histologic findings in donor cells.
MRI proved useful in transplantation of adipose-derived stem cells in vivo. This imaging technique may be useful for studies of CNS stem cell therapies. |
| doi_str_mv | 10.2214/AJR.06.0663 |
| format | Article |
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Adipose-derived stem cells harvested from mice inbred for green fluorescent protein were labeled with SPIO ferumoxide particles through the use of poly-L-lysine. Adipose-derived stem cell viability, iron staining, and proliferation were measured after SPIO labeling, and the sensitivity of MRI in the detection of SPIO-labeled adipose-derived stem cells was assessed ex vivo. Adult mice (n = 12) were subjected to unilateral MCAO. Two weeks later, in vivo 7-T MRI was performed to guide stereotactic transplantation of SPIO-labeled adipose-derived stem cells into brain tissue adjacent to the infarct. After 24 hours, the mice were sacrificed for high-resolution ex vivo 7-T or 9.4-T MRI and histologic study.
Adipose-derived stem cells were efficiently labeled with SPIO particles without loss of cell viability or proliferation. Using MRI, we guided precise transplantation of adipose-derived stem cells. MR images of mice given injections of SPIO-labeled adipose-derived stem cells had hypointense regions that correlated with the histologic findings in donor cells.
MRI proved useful in transplantation of adipose-derived stem cells in vivo. This imaging technique may be useful for studies of CNS stem cell therapies.</description><identifier>ISSN: 0361-803X</identifier><identifier>EISSN: 1546-3141</identifier><identifier>DOI: 10.2214/AJR.06.0663</identifier><identifier>PMID: 17377054</identifier><identifier>CODEN: AAJRDX</identifier><language>eng</language><publisher>Leesburg, VA: Am Roentgen Ray Soc</publisher><subject>Adipose Tissue - cytology ; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy ; Animals ; Biological and medical sciences ; Bone marrow, stem cells transplantation. Graft versus host reaction ; Brain Ischemia - diagnosis ; Brain Ischemia - etiology ; Brain Ischemia - surgery ; Contrast Media ; Dextrans ; Ferrosoferric Oxide ; Infarction, Middle Cerebral Artery - complications ; Iron ; Magnetic Resonance Imaging ; Magnetite Nanoparticles ; Medical sciences ; Mice ; Mice, Inbred C57BL ; Oxides ; Stem Cell Transplantation - methods ; Transfusions. Complications. Transfusion reactions. Cell and gene therapy</subject><ispartof>American journal of roentgenology (1976), 2007-04, Vol.188 (4), p.1101-1108</ispartof><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c380t-12d122d374edbe7e041861493ee5434e2f50069ed4f32e1a3bcfdd5322524f7e3</citedby><cites>FETCH-LOGICAL-c380t-12d122d374edbe7e041861493ee5434e2f50069ed4f32e1a3bcfdd5322524f7e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4105,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18646909$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17377054$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rice, Henry E</creatorcontrib><creatorcontrib>Hsu, Edward W</creatorcontrib><creatorcontrib>Sheng, Huaxin</creatorcontrib><creatorcontrib>Evenson, Debra A</creatorcontrib><creatorcontrib>Freemerman, Alex J</creatorcontrib><creatorcontrib>Safford, Kristine M</creatorcontrib><creatorcontrib>Provenzale, James M</creatorcontrib><creatorcontrib>Warner, David S</creatorcontrib><creatorcontrib>Johnson, G. Allan</creatorcontrib><title>Superparamagnetic Iron Oxide Labeling and Transplantation of Adipose-Derived Stem Cells in Middle Cerebral Artery Occlusion-Injured Mice</title><title>American journal of roentgenology (1976)</title><addtitle>AJR Am J Roentgenol</addtitle><description>Adipose-derived stem cells are an alternative stem cell source for CNS therapies. The goals of the current study were to label adipose-derived stem cells with superparamagnetic iron oxide (SPIO) particles, to use MRI to guide the transplantation of adipose-derived stem cells in middle cerebral artery occlusion (MCAO)-injured mice, and to localize donor adipose-derived stem cells in the injured brain using MRI. We hypothesized that we would successfully label adipose-derived stem cells and image them with MRI.
Adipose-derived stem cells harvested from mice inbred for green fluorescent protein were labeled with SPIO ferumoxide particles through the use of poly-L-lysine. Adipose-derived stem cell viability, iron staining, and proliferation were measured after SPIO labeling, and the sensitivity of MRI in the detection of SPIO-labeled adipose-derived stem cells was assessed ex vivo. Adult mice (n = 12) were subjected to unilateral MCAO. Two weeks later, in vivo 7-T MRI was performed to guide stereotactic transplantation of SPIO-labeled adipose-derived stem cells into brain tissue adjacent to the infarct. After 24 hours, the mice were sacrificed for high-resolution ex vivo 7-T or 9.4-T MRI and histologic study.
Adipose-derived stem cells were efficiently labeled with SPIO particles without loss of cell viability or proliferation. Using MRI, we guided precise transplantation of adipose-derived stem cells. MR images of mice given injections of SPIO-labeled adipose-derived stem cells had hypointense regions that correlated with the histologic findings in donor cells.
MRI proved useful in transplantation of adipose-derived stem cells in vivo. This imaging technique may be useful for studies of CNS stem cell therapies.</description><subject>Adipose Tissue - cytology</subject><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Bone marrow, stem cells transplantation. Graft versus host reaction</subject><subject>Brain Ischemia - diagnosis</subject><subject>Brain Ischemia - etiology</subject><subject>Brain Ischemia - surgery</subject><subject>Contrast Media</subject><subject>Dextrans</subject><subject>Ferrosoferric Oxide</subject><subject>Infarction, Middle Cerebral Artery - complications</subject><subject>Iron</subject><subject>Magnetic Resonance Imaging</subject><subject>Magnetite Nanoparticles</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Oxides</subject><subject>Stem Cell Transplantation - methods</subject><subject>Transfusions. Complications. Transfusion reactions. Cell and gene therapy</subject><issn>0361-803X</issn><issn>1546-3141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0cuKFDEUBuAgitOOrtxLNupCajy5VKpq2bS3lh4anBHchXRyqidD6jJJle28gY9thm6YpRAIge_8hPMT8prBBedMflx-_3EBKh8lnpAFK6UqBJPsKVmAUKyoQfw6Iy9SugWAqm6q5-SMVaKqoJQL8vdqHjGOJprO7HucvKXrOPR0-8c7pBuzw-D7PTW9o9fR9GkMpp_M5DMZWrp0fhwSFp8w-t_o6NWEHV1hCIn6nl565wLmd8RdNIEu44Txnm6tDXPKCcW6v51jHrv0Fl-SZ60JCV-d7nPy88vn69W3YrP9ul4tN4UVNUwF445x7kQl0e2wQpCsVkw2ArGUQiJvSwDVoJOt4MiM2NnWuVJwXnLZVijOybtj7hiHuxnTpDufbP6y6XGYk66AN3lL6r-Qg6oFKJnhhyO0cUgpYqvH6DsT7zUD_dCQzg1pUPqhoazfnGLnXYfu0Z4qyeDtCZhkTWjz1q1Pj65WUjXQZPf-6G78_ubgI-rUmRByLNOHw4HVtZaaMWDiH5qIptI</recordid><startdate>20070401</startdate><enddate>20070401</enddate><creator>Rice, Henry E</creator><creator>Hsu, Edward W</creator><creator>Sheng, Huaxin</creator><creator>Evenson, Debra A</creator><creator>Freemerman, Alex J</creator><creator>Safford, Kristine M</creator><creator>Provenzale, James M</creator><creator>Warner, David S</creator><creator>Johnson, G. Allan</creator><general>Am Roentgen Ray Soc</general><general>American Roentgen Ray Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20070401</creationdate><title>Superparamagnetic Iron Oxide Labeling and Transplantation of Adipose-Derived Stem Cells in Middle Cerebral Artery Occlusion-Injured Mice</title><author>Rice, Henry E ; Hsu, Edward W ; Sheng, Huaxin ; Evenson, Debra A ; Freemerman, Alex J ; Safford, Kristine M ; Provenzale, James M ; Warner, David S ; Johnson, G. Allan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c380t-12d122d374edbe7e041861493ee5434e2f50069ed4f32e1a3bcfdd5322524f7e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Adipose Tissue - cytology</topic><topic>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Bone marrow, stem cells transplantation. Graft versus host reaction</topic><topic>Brain Ischemia - diagnosis</topic><topic>Brain Ischemia - etiology</topic><topic>Brain Ischemia - surgery</topic><topic>Contrast Media</topic><topic>Dextrans</topic><topic>Ferrosoferric Oxide</topic><topic>Infarction, Middle Cerebral Artery - complications</topic><topic>Iron</topic><topic>Magnetic Resonance Imaging</topic><topic>Magnetite Nanoparticles</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Oxides</topic><topic>Stem Cell Transplantation - methods</topic><topic>Transfusions. Complications. Transfusion reactions. Cell and gene therapy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rice, Henry E</creatorcontrib><creatorcontrib>Hsu, Edward W</creatorcontrib><creatorcontrib>Sheng, Huaxin</creatorcontrib><creatorcontrib>Evenson, Debra A</creatorcontrib><creatorcontrib>Freemerman, Alex J</creatorcontrib><creatorcontrib>Safford, Kristine M</creatorcontrib><creatorcontrib>Provenzale, James M</creatorcontrib><creatorcontrib>Warner, David S</creatorcontrib><creatorcontrib>Johnson, G. 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Allan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Superparamagnetic Iron Oxide Labeling and Transplantation of Adipose-Derived Stem Cells in Middle Cerebral Artery Occlusion-Injured Mice</atitle><jtitle>American journal of roentgenology (1976)</jtitle><addtitle>AJR Am J Roentgenol</addtitle><date>2007-04-01</date><risdate>2007</risdate><volume>188</volume><issue>4</issue><spage>1101</spage><epage>1108</epage><pages>1101-1108</pages><issn>0361-803X</issn><eissn>1546-3141</eissn><coden>AAJRDX</coden><abstract>Adipose-derived stem cells are an alternative stem cell source for CNS therapies. The goals of the current study were to label adipose-derived stem cells with superparamagnetic iron oxide (SPIO) particles, to use MRI to guide the transplantation of adipose-derived stem cells in middle cerebral artery occlusion (MCAO)-injured mice, and to localize donor adipose-derived stem cells in the injured brain using MRI. We hypothesized that we would successfully label adipose-derived stem cells and image them with MRI.
Adipose-derived stem cells harvested from mice inbred for green fluorescent protein were labeled with SPIO ferumoxide particles through the use of poly-L-lysine. Adipose-derived stem cell viability, iron staining, and proliferation were measured after SPIO labeling, and the sensitivity of MRI in the detection of SPIO-labeled adipose-derived stem cells was assessed ex vivo. Adult mice (n = 12) were subjected to unilateral MCAO. Two weeks later, in vivo 7-T MRI was performed to guide stereotactic transplantation of SPIO-labeled adipose-derived stem cells into brain tissue adjacent to the infarct. After 24 hours, the mice were sacrificed for high-resolution ex vivo 7-T or 9.4-T MRI and histologic study.
Adipose-derived stem cells were efficiently labeled with SPIO particles without loss of cell viability or proliferation. Using MRI, we guided precise transplantation of adipose-derived stem cells. MR images of mice given injections of SPIO-labeled adipose-derived stem cells had hypointense regions that correlated with the histologic findings in donor cells.
MRI proved useful in transplantation of adipose-derived stem cells in vivo. This imaging technique may be useful for studies of CNS stem cell therapies.</abstract><cop>Leesburg, VA</cop><pub>Am Roentgen Ray Soc</pub><pmid>17377054</pmid><doi>10.2214/AJR.06.0663</doi><tpages>8</tpages></addata></record> |
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| ispartof | American journal of roentgenology (1976), 2007-04, Vol.188 (4), p.1101-1108 |
| issn | 0361-803X 1546-3141 |
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| source | American Roentgen Ray Society; MEDLINE; Alma/SFX Local Collection |
| subjects | Adipose Tissue - cytology Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Animals Biological and medical sciences Bone marrow, stem cells transplantation. Graft versus host reaction Brain Ischemia - diagnosis Brain Ischemia - etiology Brain Ischemia - surgery Contrast Media Dextrans Ferrosoferric Oxide Infarction, Middle Cerebral Artery - complications Iron Magnetic Resonance Imaging Magnetite Nanoparticles Medical sciences Mice Mice, Inbred C57BL Oxides Stem Cell Transplantation - methods Transfusions. Complications. Transfusion reactions. Cell and gene therapy |
| title | Superparamagnetic Iron Oxide Labeling and Transplantation of Adipose-Derived Stem Cells in Middle Cerebral Artery Occlusion-Injured Mice |
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