Oligothiophene Molecular Beacons
Oligomers of thiophene are widely studied compounds for their electronic and optoelectronic properties. Despite their strong fluorescence, their use as markers for biomolecules, especially for oligonucleotides (ONs), is still largely unexplored. Here, we describe the synthesis of a series of ON mole...
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| Veröffentlicht in: | Bioconjugate chemistry 2007-03, Vol.18 (2), p.318-322 |
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| container_end_page | 322 |
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| container_issue | 2 |
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| container_title | Bioconjugate chemistry |
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| creator | Cazzato, Antonella Capobianco, Massimo L Zambianchi, Massimo Favaretto, Laura Bettini, Cristian Barbarella, Giovanna |
| description | Oligomers of thiophene are widely studied compounds for their electronic and optoelectronic properties. Despite their strong fluorescence, their use as markers for biomolecules, especially for oligonucleotides (ONs), is still largely unexplored. Here, we describe the synthesis of a series of ON molecular beacons employing different oligothiophenes as fluorescent probes and discuss their fluorescence emissions in comparative experiments with and without dabcyl as a quencher, in their hairpin and linear conformations, and as duplexes after hybridization with a complementary target. |
| doi_str_mv | 10.1021/bc060169n |
| format | Article |
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| ispartof | Bioconjugate chemistry, 2007-03, Vol.18 (2), p.318-322 |
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| language | eng |
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| source | MEDLINE; ACS Publications |
| subjects | Biochemistry Chemical compounds Comparative analysis Electric properties Fluorescence Fluorescent Dyes - chemistry Fluorescent Dyes - metabolism Hybridization Models, Molecular Molecules Nucleic Acid Conformation Nucleic Acid Hybridization Oligonucleotide Probes - chemistry Oligonucleotide Probes - metabolism Oligonucleotides - chemistry Spectrometry, Fluorescence Thiophenes - chemistry Thiophenes - metabolism |
| title | Oligothiophene Molecular Beacons |
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