Oligothiophene Molecular Beacons

Oligomers of thiophene are widely studied compounds for their electronic and optoelectronic properties. Despite their strong fluorescence, their use as markers for biomolecules, especially for oligonucleotides (ONs), is still largely unexplored. Here, we describe the synthesis of a series of ON mole...

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Veröffentlicht in:Bioconjugate chemistry 2007-03, Vol.18 (2), p.318-322
Hauptverfasser: Cazzato, Antonella, Capobianco, Massimo L, Zambianchi, Massimo, Favaretto, Laura, Bettini, Cristian, Barbarella, Giovanna
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Sprache:eng
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Zusammenfassung:Oligomers of thiophene are widely studied compounds for their electronic and optoelectronic properties. Despite their strong fluorescence, their use as markers for biomolecules, especially for oligonucleotides (ONs), is still largely unexplored. Here, we describe the synthesis of a series of ON molecular beacons employing different oligothiophenes as fluorescent probes and discuss their fluorescence emissions in comparative experiments with and without dabcyl as a quencher, in their hairpin and linear conformations, and as duplexes after hybridization with a complementary target.
ISSN:1043-1802
1520-4812
DOI:10.1021/bc060169n