DNA Introduction into Living Cells by Water Droplet Impact with an Electrospray Process

DNA Introduction into Living Cells by Water Droplet Impact with an Electrospray Process

Charged water droplets from an electrospray device can generate a transient hole in cell membranes for DNA transport into a living cell. The number of cells containing DNA encoded with green fluorescent protein (GFP) increases with an increase in applied voltage. The technique can be applied to gene...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Angewandte Chemie (International ed.) 2008-02, Vol.47 (8), p.1429-1431
Hauptverfasser: Okubo, Yusuke, Ikemoto, Kazuto, Koike, Kanako, Tsutsui, Chihiro, Sakata, Ichiro, Takei, Osamu, Adachi, Akihito, Sakai, Takafumi
Format: Artikel
Sprache:eng
Schlagworte:
aerosols
Animals
Cell Line
Cell Survival
Cricetinae
Cricetulus
DNA
DNA - chemistry
DNA - genetics
drug delivery
Electrons
electrospray
gene expression
Gene Transfer Techniques
Humans
Transgenes - genetics
Water - chemistry
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Charged water droplets from an electrospray device can generate a transient hole in cell membranes for DNA transport into a living cell. The number of cells containing DNA encoded with green fluorescent protein (GFP) increases with an increase in applied voltage. The technique can be applied to gene transfection to eukaryotic cells, tissues, and prokaryotic cells.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.200704429