Simultaneous use of multiple fluorescent reporter dyes for glucose sensing in aqueous solution

The simultaneous use of several fluorescent reporter dyes in a multicomponent boronic acid-based glucose sensing system is reported. In one application, two dyes with widely different emission wavelengths are used to report changes in glucose concentration. A third glucose-insensitive dye was then a...

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Veröffentlicht in:Analytical and bioanalytical chemistry 2007-04, Vol.387 (8), p.2767-2773
Hauptverfasser: Cordes, David B, Miller, Aaron, Gamsey, Soya, Singaram, Bakthan
Format: Artikel
Sprache:eng
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Zusammenfassung:The simultaneous use of several fluorescent reporter dyes in a multicomponent boronic acid-based glucose sensing system is reported. In one application, two dyes with widely different emission wavelengths are used to report changes in glucose concentration. A third glucose-insensitive dye was then added to act as a reference dye and provide for a ratiometric correction to the two reporter dye signals. The inclusion of such a reference dye reduces errors arising from sources such as fluctuations in lamp intensity and sample dilution.
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-007-1128-z