Delivery of Nitric Oxide for Analysis of the Function of Cytochrome c

On delivery of nitric oxide (NO) to protein samples (e.g., cytochrome c′), for spectroscopic experiments it is important to avoid exposure to oxygen and to remove contaminants from the NO gas. We describe a number of techniques for steady‐state UV/Vis spectrophotometry and pre‐steady‐state stopped‐f...

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Hauptverfasser: Cole, Lindsay J., Huston, Wilhelmina M., Moir, James W.B.
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Huston, Wilhelmina M.
Moir, James W.B.
description On delivery of nitric oxide (NO) to protein samples (e.g., cytochrome c′), for spectroscopic experiments it is important to avoid exposure to oxygen and to remove contaminants from the NO gas. We describe a number of techniques for steady‐state UV/Vis spectrophotometry and pre‐steady‐state stopped‐flow spectrophotometry analysis of cytochrome c′.
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subjects Cytochromes c' - chemistry
Cytochromes c' - metabolism
Ferrous Compounds - chemistry
Ferrous Compounds - metabolism
Heme - chemistry
Neisseria meningitidis - metabolism
Nitric Oxide - metabolism
Rhodobacter capsulatus - metabolism
Spectrophotometry
Spectrophotometry, Ultraviolet
title Delivery of Nitric Oxide for Analysis of the Function of Cytochrome c
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