Delivery of Nitric Oxide for Analysis of the Function of Cytochrome c
On delivery of nitric oxide (NO) to protein samples (e.g., cytochrome c′), for spectroscopic experiments it is important to avoid exposure to oxygen and to remove contaminants from the NO gas. We describe a number of techniques for steady‐state UV/Vis spectrophotometry and pre‐steady‐state stopped‐f...
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creator | Cole, Lindsay J. Huston, Wilhelmina M. Moir, James W.B. |
description | On delivery of nitric oxide (NO) to protein samples (e.g., cytochrome c′), for spectroscopic experiments it is important to avoid exposure to oxygen and to remove contaminants from the NO gas. We describe a number of techniques for steady‐state UV/Vis spectrophotometry and pre‐steady‐state stopped‐flow spectrophotometry analysis of cytochrome c′. |
doi_str_mv | 10.1016/S0076-6879(08)36002-9 |
format | Book Chapter |
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We describe a number of techniques for steady‐state UV/Vis spectrophotometry and pre‐steady‐state stopped‐flow spectrophotometry analysis of cytochrome c′.</description><identifier>ISSN: 0076-6879</identifier><identifier>ISBN: 0123742773</identifier><identifier>ISBN: 9780123742773</identifier><identifier>EISSN: 1557-7988</identifier><identifier>DOI: 10.1016/S0076-6879(08)36002-9</identifier><identifier>PMID: 18237625</identifier><language>eng</language><publisher>United States: Elsevier Science & Technology</publisher><subject>Cytochromes c' - chemistry ; Cytochromes c' - metabolism ; Ferrous Compounds - chemistry ; Ferrous Compounds - metabolism ; Heme - chemistry ; Neisseria meningitidis - metabolism ; Nitric Oxide - metabolism ; Rhodobacter capsulatus - metabolism ; Spectrophotometry ; Spectrophotometry, Ultraviolet</subject><ispartof>Methods in Enzymology, 2008, Vol.436, p.21-33</ispartof><rights>2008 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0076687908360029$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,779,780,784,793,3459,3550,4024,11288,27923,27924,27925,45810,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18237625$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cole, Lindsay J.</creatorcontrib><creatorcontrib>Huston, Wilhelmina M.</creatorcontrib><creatorcontrib>Moir, James W.B.</creatorcontrib><title>Delivery of Nitric Oxide for Analysis of the Function of Cytochrome c</title><title>Methods in Enzymology</title><addtitle>Methods Enzymol</addtitle><description>On delivery of nitric oxide (NO) to protein samples (e.g., cytochrome c′), for spectroscopic experiments it is important to avoid exposure to oxygen and to remove contaminants from the NO gas. We describe a number of techniques for steady‐state UV/Vis spectrophotometry and pre‐steady‐state stopped‐flow spectrophotometry analysis of cytochrome c′.</description><subject>Cytochromes c' - chemistry</subject><subject>Cytochromes c' - metabolism</subject><subject>Ferrous Compounds - chemistry</subject><subject>Ferrous Compounds - metabolism</subject><subject>Heme - chemistry</subject><subject>Neisseria meningitidis - metabolism</subject><subject>Nitric Oxide - metabolism</subject><subject>Rhodobacter capsulatus - metabolism</subject><subject>Spectrophotometry</subject><subject>Spectrophotometry, Ultraviolet</subject><issn>0076-6879</issn><issn>1557-7988</issn><isbn>0123742773</isbn><isbn>9780123742773</isbn><fulltext>true</fulltext><rsrctype>book_chapter</rsrctype><creationdate>2008</creationdate><recordtype>book_chapter</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE1PwzAMhiM-xMbYTwD1hOBQSNI2aU9oGhsgTewAnKM2cbSgthlJO9F_TzsGvliWH1l-H4QuCb4jmLD7N4w5C1nKsxuc3kYMYxpmR2hMkoSHPEvTY3SOCY14TDmPTtD4nx-hqfefuC_CoozwMzQiaQ8ymozR4hFKswPXBVYHr6ZxRgbrb6Mg0NYFszovO2_8sGw2ECzbWjbG1sM87xorN85WEMgLdKrz0sP00CfoY7l4nz-Hq_XTy3y2CoGypAl1GmusKKUyi6jmSgGLJS0KlhQRZUxnQHJKGGipeBwDaD3E5FLSuEhxoqIJuv69u3X2qwXfiMp4CWWZ12BbLzimSRxj2oNXB7AtKlBi60yVu078Be-Bh18A-nd3Bpzw0kAtQRkHshHKGkGwGNSLvXoxqBQ4FXv1Iot-APYrcbk</recordid><startdate>2008</startdate><enddate>2008</enddate><creator>Cole, Lindsay J.</creator><creator>Huston, Wilhelmina M.</creator><creator>Moir, James W.B.</creator><general>Elsevier Science & Technology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>2008</creationdate><title>Delivery of Nitric Oxide for Analysis of the Function of Cytochrome c</title><author>Cole, Lindsay J. ; Huston, Wilhelmina M. ; Moir, James W.B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e265t-f84f0d222c932f7dde64c2bb65b3266f9e1a216efcd744eeff60027cc24b805d3</frbrgroupid><rsrctype>book_chapters</rsrctype><prefilter>book_chapters</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Cytochromes c' - chemistry</topic><topic>Cytochromes c' - metabolism</topic><topic>Ferrous Compounds - chemistry</topic><topic>Ferrous Compounds - metabolism</topic><topic>Heme - chemistry</topic><topic>Neisseria meningitidis - metabolism</topic><topic>Nitric Oxide - metabolism</topic><topic>Rhodobacter capsulatus - metabolism</topic><topic>Spectrophotometry</topic><topic>Spectrophotometry, Ultraviolet</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cole, Lindsay J.</creatorcontrib><creatorcontrib>Huston, Wilhelmina M.</creatorcontrib><creatorcontrib>Moir, James W.B.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cole, Lindsay J.</au><au>Huston, Wilhelmina M.</au><au>Moir, James W.B.</au><format>book</format><genre>bookitem</genre><ristype>CHAP</ristype><atitle>Delivery of Nitric Oxide for Analysis of the Function of Cytochrome c</atitle><btitle>Methods in Enzymology</btitle><addtitle>Methods Enzymol</addtitle><date>2008</date><risdate>2008</risdate><volume>436</volume><spage>21</spage><epage>33</epage><pages>21-33</pages><issn>0076-6879</issn><eissn>1557-7988</eissn><isbn>0123742773</isbn><isbn>9780123742773</isbn><abstract>On delivery of nitric oxide (NO) to protein samples (e.g., cytochrome c′), for spectroscopic experiments it is important to avoid exposure to oxygen and to remove contaminants from the NO gas. We describe a number of techniques for steady‐state UV/Vis spectrophotometry and pre‐steady‐state stopped‐flow spectrophotometry analysis of cytochrome c′.</abstract><cop>United States</cop><pub>Elsevier Science & Technology</pub><pmid>18237625</pmid><doi>10.1016/S0076-6879(08)36002-9</doi><tpages>13</tpages></addata></record> |
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source | MEDLINE; ScienceDirect eBooks; Access via ScienceDirect (Elsevier) |
subjects | Cytochromes c' - chemistry Cytochromes c' - metabolism Ferrous Compounds - chemistry Ferrous Compounds - metabolism Heme - chemistry Neisseria meningitidis - metabolism Nitric Oxide - metabolism Rhodobacter capsulatus - metabolism Spectrophotometry Spectrophotometry, Ultraviolet |
title | Delivery of Nitric Oxide for Analysis of the Function of Cytochrome c |
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