Delivery of Nitric Oxide for Analysis of the Function of Cytochrome c

Delivery of Nitric Oxide for Analysis of the Function of Cytochrome c

On delivery of nitric oxide (NO) to protein samples (e.g., cytochrome c′), for spectroscopic experiments it is important to avoid exposure to oxygen and to remove contaminants from the NO gas. We describe a number of techniques for steady‐state UV/Vis spectrophotometry and pre‐steady‐state stopped‐f...

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Bibliographische Detailangaben
Hauptverfasser: Cole, Lindsay J., Huston, Wilhelmina M., Moir, James W.B.
Format: Buchkapitel
Sprache:eng
Schlagworte:
Cytochromes c' - chemistry
Cytochromes c' - metabolism
Ferrous Compounds - chemistry
Ferrous Compounds - metabolism
Heme - chemistry
Neisseria meningitidis - metabolism
Nitric Oxide - metabolism
Rhodobacter capsulatus - metabolism
Spectrophotometry
Spectrophotometry, Ultraviolet
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Zusammenfassung:On delivery of nitric oxide (NO) to protein samples (e.g., cytochrome c′), for spectroscopic experiments it is important to avoid exposure to oxygen and to remove contaminants from the NO gas. We describe a number of techniques for steady‐state UV/Vis spectrophotometry and pre‐steady‐state stopped‐flow spectrophotometry analysis of cytochrome c′.
ISSN:0076-6879
1557-7988
DOI:10.1016/S0076-6879(08)36002-9