Fluorescent Stain Method for the Simultaneous Determination of Mitochondrial Potential and Integrity of Plasma and Acrosomal Membranes in Boar Sperm

The purpose of this study was to validate a technique for simultaneous evaluation of the plasma, acrosomal and mitochondrial membranes in boar spermatozoa, using an association of fluorescent probes: Propidium iodide (PI), fluorescein isothiocyanate-conjugated Pisum sativum agglutinin (FITC-PSA) and...

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Veröffentlicht in:	Reproduction in domestic animals 2007-04, Vol.42 (2), p.190-194
Hauptverfasser:	de Andrade, AFC, de Arruda, RP, Celeghini, ECC, Nascimento, J, Martins, SMMK, Raphael, CF, Moretti, AS
Format:	Artikel
Sprache:	eng
Schlagworte:	Acrosome - physiology Acrosome - ultrastructure Animals Biological and medical sciences Body fluids Cell Membrane - physiology Cell Membrane - ultrastructure Fluorescein-5-isothiocyanate Fluorescence Fundamental and applied biological sciences. Psychology Hogs Male Mammalian reproduction. General aspects Membranes Microscopy, Fluorescence - veterinary Mitochondria - metabolism Mitochondria - physiology Mitochondria - ultrastructure Mitochondrial DNA Plasma Propidium Reproductive system Sperm Motility - physiology Spermatozoa - abnormalities Spermatozoa - physiology Swine - physiology Vertebrates: reproduction
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Beschreibung
Zusammenfassung:	The purpose of this study was to validate a technique for simultaneous evaluation of the plasma, acrosomal and mitochondrial membranes in boar spermatozoa, using an association of fluorescent probes: Propidium iodide (PI), fluorescein isothiocyanate-conjugated Pisum sativum agglutinin (FITC-PSA) and JC-1. Three ejaculates from each of four different boars, all showing motility >=80% and abnormal morphology
ISSN:	0936-6768 1439-0531
DOI:	10.1111/j.1439-0531.2006.00751.x