Bacillus butanolivorans sp. nov., a species with industrial application for the remediation of n-butanol

1 Department of Plant Physiology and Microbiology, Vilnius University, Chiurlionio 21/27, Vilnius LT-03101, Lithuania 2 DSMZ – Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstraße 7b, 38124 Braunschweig, Germany Correspondence Nomeda Kuisiene nomeda.kuisiene{at}gf.vu.lt Four b...

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Veröffentlicht in:International journal of systematic and evolutionary microbiology 2008-02, Vol.58 (2), p.505-509
Hauptverfasser: Kuisiene, Nomeda, Raugalas, Juozas, Sproer, Cathrin, Kroppenstedt, Reiner M, Chitavichius, Donaldas
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Sprache:eng
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Zusammenfassung:1 Department of Plant Physiology and Microbiology, Vilnius University, Chiurlionio 21/27, Vilnius LT-03101, Lithuania 2 DSMZ – Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstraße 7b, 38124 Braunschweig, Germany Correspondence Nomeda Kuisiene nomeda.kuisiene{at}gf.vu.lt Four bacterial strains, designated K9 T , K105, K1012A and K101, were isolated from soil in Lithuania. All these strains could use n-butanol as a sole carbon source. The strains grew in a medium containing 12–120 mM n-butanol. The strains were strictly aerobic, Gram-positive endospore-formers. The best growth was achieved at 25 °C and pH 7.0 in medium containing 1 % (w/v) NaCl. The strains showed identical profiles of 16S–23S rRNA internal transcribed spacer PCR and nearly identical 16S rRNA gene PCR-RFLP electrophoretic patterns and physiological characteristics, demonstrating their relationship at the species level. The cellular fatty acid profile of K9 T consisted of significant amounts of the C 15 branched-chain fatty acids iso-C 15 : 0 (16.78 %) and anteiso-C 15 : 0 (45.80 %). The diagnostic cell-wall diamino acid was meso -diaminopimelic acid. The 16S rRNA gene sequence of K9 T showed the highest similarity to the sequences of Bacillus simplex DSM 1321 T and Bacillus muralis LMG 20238 T (98.3 and 97.7 %, respectively). The DNA G+C content was 37.4 mol%. Studies of DNA–DNA relatedness, morphological, physiological and chemotaxonomic analyses and phylogenetic data based on 16S rRNA gene sequencing allowed strains K9 T , K105, K1012A and K101 to be described as members of a novel species of the genus Bacillus , for which the name Bacillus butanolivorans sp. nov. is proposed. The type strain is K9 T (=DSM 18926 T =LMG 23974 T ). Abbreviations: ARDRA, amplified rDNA restriction analysis; RISA, ribosomal intergenic spacer analysis The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain K9 T is EF206294 . ARDRA and RISA profiles for the novel strains are available as supplementary material with the online version of this paper.
ISSN:1466-5026
1466-5034
DOI:10.1099/ijs.0.65332-0