Using coupled monolithic rods for ultra-high peak capacity LC and LC-MS under normal LC operating pressures

Up to ten individual reversed-phase monolithic LC columns were efficiently coupled to equal a total column length of 1 m. The column generated up to 90,000 theoretical plates under optimum conditions with a peak capacity under gradient conditions of 244 peaks h(-1) at a total column backpressure of...

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Veröffentlicht in:	Analyst (London) 2008-02, Vol.133 (2), p.180-183
Hauptverfasser:	BONES, Jonathan, DUFFY, Cepta, MACKA, Mirek, PAULL, Brett
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Animals Chemistry Chromatographic methods and physical methods associated with chromatography Chromatography, Liquid - instrumentation Chromatography, Liquid - methods Exact sciences and technology Humans Other chromatographic methods Proteins - analysis Sensitivity and Specificity Spectrometric and optical methods Spectrometry, Mass, Electrospray Ionization - methods
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	Up to ten individual reversed-phase monolithic LC columns were efficiently coupled to equal a total column length of 1 m. The column generated up to 90,000 theoretical plates under optimum conditions with a peak capacity under gradient conditions of 244 peaks h(-1) at a total column backpressure of just 140 bar, directly coupled to ESI-MS.
ISSN:	0003-2654 1364-5528
DOI:	10.1039/b714807k