Application of Thiolated Gold Nanoparticles for the Enhancement of Glucose Oxidase Activity

Glucose oxidase (GOx) has been covalently immobilized onto chemically synthesized thiolated gold nanoparticles (5−8 nm) via N-ethyl-N‘-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS). The lower value of the Michaelis−Menton constant obtained for the immobilized (3.74 mM) GO...

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Veröffentlicht in:	Langmuir 2007-03, Vol.23 (6), p.3333-3337
Hauptverfasser:	Pandey, Pratibha, Singh, Surinder P, Arya, Sunil K, Gupta, Vinay, Datta, Monika, Singh, Sukhvir, Malhotra, Bansi D
Format:	Artikel
Sprache:	eng
Schlagworte:	Biosensing Techniques Chemistry Chemistry, Physical - methods Colloidal state and disperse state Enzymes, Immobilized - chemistry Exact sciences and technology General and physical chemistry Glucose Oxidase - chemistry Gold - chemistry Hydrogen-Ion Concentration Kinetics Metal Nanoparticles - chemistry Microscopy, Electron, Transmission Models, Chemical Physical and chemical studies. Granulometry. Electrokinetic phenomena Spectroscopy, Fourier Transform Infrared - methods Succinimides - chemistry Sulfhydryl Compounds - chemistry Temperature Time Factors
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