Application of Thiolated Gold Nanoparticles for the Enhancement of Glucose Oxidase Activity

Application of Thiolated Gold Nanoparticles for the Enhancement of Glucose Oxidase Activity

Glucose oxidase (GOx) has been covalently immobilized onto chemically synthesized thiolated gold nanoparticles (5−8 nm) via N-ethyl-N‘-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS). The lower value of the Michaelis−Menton constant obtained for the immobilized (3.74 mM) GO...

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Veröffentlicht in:Langmuir 2007-03, Vol.23 (6), p.3333-3337
Hauptverfasser: Pandey, Pratibha, Singh, Surinder P, Arya, Sunil K, Gupta, Vinay, Datta, Monika, Singh, Sukhvir, Malhotra, Bansi D
Format: Artikel
Sprache:eng
Schlagworte:
Biosensing Techniques
Chemistry
Chemistry, Physical - methods
Colloidal state and disperse state
Enzymes, Immobilized - chemistry
Exact sciences and technology
General and physical chemistry
Glucose Oxidase - chemistry
Gold - chemistry
Hydrogen-Ion Concentration
Kinetics
Metal Nanoparticles - chemistry
Microscopy, Electron, Transmission
Models, Chemical
Physical and chemical studies. Granulometry. Electrokinetic phenomena
Spectroscopy, Fourier Transform Infrared - methods
Succinimides - chemistry
Sulfhydryl Compounds - chemistry
Temperature
Time Factors
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Zusammenfassung:Glucose oxidase (GOx) has been covalently immobilized onto chemically synthesized thiolated gold nanoparticles (5−8 nm) via N-ethyl-N‘-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS). The lower value of the Michaelis−Menton constant obtained for the immobilized (3.74 mM) GOx compared with that for the free (5.85 mM) GOx suggests significant enhancement in the activity of GOx attached to thiolated gold nanoparticles. The covalently immobilized GOx thiolated nanoparticles exhibit a response time of 30 s, a shelf life of more than 6 months, and improved tolerance to both pH and temperature.
ISSN:0743-7463
1520-5827
DOI:10.1021/la062901c